Targeted Metabolic Profiling of Methionine Cycle Metabolites and Redox Thiol Pools in Mammalian Plasma, Cells and Urine.

biomarker glutathione homocysteine mass spectrometry methionine metabolism targeted metabolic profiling thiol

Journal

Metabolites
ISSN: 2218-1989
Titre abrégé: Metabolites
Pays: Switzerland
ID NLM: 101578790

Informations de publication

Date de publication:
18 Oct 2019
Historique:
received: 12 09 2019
revised: 13 10 2019
accepted: 15 10 2019
entrez: 23 10 2019
pubmed: 23 10 2019
medline: 23 10 2019
Statut: epublish

Résumé

The concentration of thiol and thioether metabolites in plasma has diagnostic value in genetic diseases of B-vitamin metabolism linked to methionine utilization. Among these, cysteine/cystine (Cys/CSSC) and glutathione/oxidized glutathione (GSH/GSSG) act as cellular redox buffers. A new LC-MS/MS method was developed for the simultaneous detection of cystathionine (Cysta), methionine (Met), methionine sulfoxide (MSO), creatinine and the reduced and oxidized pairs of homocysteine (Hcy/HSSH), cysteine (Cys/CSSC) and glutathione (GSH/GSSG). A one-step thiol-blocking protocol with minimal sample preparation was established to determine redox thiol pairs in plasma and cells. The concentrations of diagnostic biomarkers Hcy, Met, Cysta, and Cys in a cohort of healthy adults (

Identifiants

DOI: 10.3390/metabo9100235 PMID: 31635306 PMC: PMC6836102
pubmed: 31635306
pii: metabo9100235
doi: 10.3390/metabo9100235
pmc: PMC6836102
pii:
doi:

Types de publication

Journal Article

Langues

eng

Références

Nucleic Acids Res. 2019 Jan 8;47(D1):D1102-D1109
pubmed: 30371825
Metabolomics. 2019 May 23;15(6):83
pubmed: 31123906
Anal Bioanal Chem. 2013 Jun;405(15):5279-89
pubmed: 23604415
Circulation. 2016 Jan 26;133(4):361-9
pubmed: 26673559
Methods Mol Biol. 2019;2007:9-17
pubmed: 31148103
Metabolites. 2019 May 17;9(5):null
pubmed: 31108909
J Nutr. 1996 Apr;126(4 Suppl):1281S-4S
pubmed: 8642471
Anal Biochem. 2012 Oct 1;429(1):45-52
pubmed: 22759776
J Cell Physiol. 1991 Jan;146(1):52-62
pubmed: 1990019
Anal Biochem. 1989 Apr;178(1):208-14
pubmed: 2729575
Science. 1964 Mar 27;143(3613):1443-5
pubmed: 14107447
Annu Rev Nutr. 2015;35:33-70
pubmed: 25974692
Clin Chem. 1994 Jun;40(6):873-81
pubmed: 8087981
J Proteome Res. 2011 Feb 4;10(2):592-603
pubmed: 21053925
Biochem Biophys Res Commun. 1972 Jan 31;46(2):905-12
pubmed: 5057914
J Nutr. 2015 Jul;145(7):1636S-1680S
pubmed: 26451605
Free Radic Biol Med. 2000 Feb 15;28(4):625-35
pubmed: 10719244
Immunity. 2017 Apr 18;46(4):525-527
pubmed: 28423332
Mol Genet Metab. 2011 Jul;103(3):226-39
pubmed: 21497120
J Proteome Res. 2018 Jan 5;17(1):203-211
pubmed: 29064256
Pediatr Res. 1994 Jan;35(1):3-9
pubmed: 8134195
Mass Spectrom Rev. 2011 May-Jun;30(3):491-509
pubmed: 21500246
J Biol Chem. 2013 Sep 13;288(37):26497-504
pubmed: 23861399
Anal Biochem. 2003 Feb 1;313(1):28-33
pubmed: 12576054
J Biol Chem. 2012 Feb 10;287(7):4397-402
pubmed: 22147695
Anal Bioanal Chem. 2011 Jan;399(3):1127-39
pubmed: 21125262
Arch Biochem Biophys. 1993 Jun;303(2):355-60
pubmed: 8099783
Free Radic Biol Med. 2013 Dec;65:244-253
pubmed: 23747983
Anal Sci. 2018;34(4):505-508
pubmed: 29643316
J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Aug 15;1092:168-178
pubmed: 29906678
Metabolomics. 2018;14(1):18
pubmed: 29354024
J Biol Chem. 1980 Oct 25;255(20):9530-3
pubmed: 7430084
J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Oct 15;877(28):3274-81
pubmed: 19515618
J Cell Biol. 2018 Jul 2;217(7):2291-2298
pubmed: 29915025
J Nutr. 2017 Jun;147(6):1167-1173
pubmed: 28381531
Redox Biol. 2018 Jun;16:359-380
pubmed: 29627744
Immunity. 2017 Apr 18;46(4):675-689
pubmed: 28423341
Elife. 2019 Mar 27;8:
pubmed: 30916644
Hum Mutat. 2010 Aug;31(8):924-9
pubmed: 20524213
Nature. 2019 Aug;572(7769):397-401
pubmed: 31367041
Physiol Rev. 2019 Jan 1;99(1):555-604
pubmed: 30427275
Hum Mol Genet. 2015 Dec 15;24(24):7049-59
pubmed: 26420839
Anal Bioanal Chem. 2016 Jan;408(3):747-60
pubmed: 26537926
Clin Chim Acta. 2008 Oct;396(1-2):43-8
pubmed: 18634771
Metabolites. 2019 Mar 05;9(3):null
pubmed: 30841653
Anal Chim Acta. 2019 Nov 8;1080:127-137
pubmed: 31409462
Biochimie. 2013 May;95(5):1023-32
pubmed: 23415655
Elife. 2019 May 02;8:
pubmed: 31043227
Sci Rep. 2018 Jan 17;8(1):932
pubmed: 29343798
Anal Chem. 2007 Sep 15;79(18):7131-8
pubmed: 17705399
J Pharm Biomed Anal. 2015 Jun 10;110:83-92
pubmed: 25818703
Crit Rev Oncol Hematol. 2018 Aug;128:43-57
pubmed: 29958630
Anal Chem. 2003 Jul 1;75(13):3019-30
pubmed: 12964746
Clin Chim Acta. 1978 Apr 17;85(2):167-73
pubmed: 647978
J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Dec 1;972:14-21
pubmed: 25306114
Cell Metab. 2017 Jan 10;25(1):27-42
pubmed: 27641100
Int J Cancer. 2019 Mar 1;144(5):947-956
pubmed: 29786139
Anal Chem. 2017 Sep 5;89(17):9424-9431
pubmed: 28787119
Nat Rev Dis Primers. 2017 Jun 29;3:17040
pubmed: 28660890
Front Mol Biosci. 2016 Jun 27;3:27
pubmed: 27446930
Methods Mol Biol. 2019;1967:197-210
pubmed: 31069772
Clin Chim Acta. 1998 Jul 28;275(2):175-84
pubmed: 9721075

Auteurs

Sidney Behringer (S)

Laboratory of Clinical Biochemistry and Metabolism, Department of General Pediatrics, Adolescent Medicine and Neonatology, Faculty of Medicine, Medical Center, University of Freiburg, 79106 Freiburg, Germany. sidney.behringer@uniklinik-freiburg.de.

Victoria Wingert (V)

Laboratory of Clinical Biochemistry and Metabolism, Department of General Pediatrics, Adolescent Medicine and Neonatology, Faculty of Medicine, Medical Center, University of Freiburg, 79106 Freiburg, Germany. victoria.wingert@uniklinik-freiburg.de.

Victor Oria (V)

Institute of Surgical Pathology, Faculty of Medicine, Medical Center, University of Freiburg, 79106 Freiburg, Germany. voria87@gmail.com.
Spemann Graduate School of Biology and Medicine, Faculty of Biology, University of Freiburg, 79104 Freiburg, Germany. voria87@gmail.com.
ORCID: 0000-0001-7187-7916

Anke Schumann (A)

Laboratory of Clinical Biochemistry and Metabolism, Department of General Pediatrics, Adolescent Medicine and Neonatology, Faculty of Medicine, Medical Center, University of Freiburg, 79106 Freiburg, Germany. anke.schumann@uniklinik-freiburg.de.
Department of General Pediatrics, Adolescent Medicine and Neonatology, Faculty of Medicine, Medical Center, University of Freiburg, 79106 Freiburg, Germany. anke.schumann@uniklinik-freiburg.de.

Sarah Grünert (S)

Department of General Pediatrics, Adolescent Medicine and Neonatology, Faculty of Medicine, Medical Center, University of Freiburg, 79106 Freiburg, Germany. sarah.gruenert@uniklinik-freiburg.de.

Artur Cieslar-Pobuda (A)

Nordic European Molecular Laboratory (EMBL) Partnership, Centre for Molecular Medicine Norway, University of Oslo, 0318 Oslo, Norway. artur.cieslar-pobuda@ncmm.uio.no.

Stefan Kölker (S)

Center for Pediatrics and Adolescent Medicine, Division of Pediatric Neurology and Metabolic Medicine, University Hospital Heidelberg, 69120 Heidelberg, Germany. Stefan.Koelker@med.uni-heidelberg.de.

Ann-Kathrin Lederer (AK)

Center for Complementary Medicine, Institute for Infection Prevention and Hospital Epidemiology, Faculty of Medicine, Medical Center, University of Freiburg, 79106 Freiburg, Germany. ann-kathrin.lederer@uniklinik-freiburg.de.
ORCID: 0000-0001-7984-9530

Donald W Jacobsen (DW)

Department of Cardiovascular and Metabolic Sciences, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44106, USA. jacobsd@ccf.org.

Judith Staerk (J)

Nordic European Molecular Laboratory (EMBL) Partnership, Centre for Molecular Medicine Norway, University of Oslo, 0318 Oslo, Norway. judith.staerk@ncmm.uio.no.
Norwegian Center for Stem Cell Research, Department of Immunology, Oslo University Hospital, 0372 Oslo, Norway. judith.staerk@ncmm.uio.no.

Oliver Schilling (O)

Institute of Surgical Pathology, Faculty of Medicine, Medical Center, University of Freiburg, 79106 Freiburg, Germany. oliver.schilling@mol-med.uni-freiburg.de.

Ute Spiekerkoetter (U)

Department of General Pediatrics, Adolescent Medicine and Neonatology, Faculty of Medicine, Medical Center, University of Freiburg, 79106 Freiburg, Germany. ute.spiekerkoetter@uniklinik-freiburg.de.

Luciana Hannibal (L)

Laboratory of Clinical Biochemistry and Metabolism, Department of General Pediatrics, Adolescent Medicine and Neonatology, Faculty of Medicine, Medical Center, University of Freiburg, 79106 Freiburg, Germany. luciana.hannibal@uniklinik-freiburg.de.
ORCID: 0000-0002-0911-5758

Classifications MeSH