Soluble tubulin is significantly enriched at mitotic centrosomes.
Animals
Caenorhabditis elegans
/ chemistry
Centrosome
/ chemistry
Cytoplasm
/ chemistry
Dimerization
Image Processing, Computer-Assisted
Imaging, Three-Dimensional
Metaphase
Microscopy, Electron
Microtubules
/ chemistry
Mitosis
Nocodazole
/ pharmacology
Polymers
/ chemistry
RNA Interference
Solubility
Tubulin
/ chemistry
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
02 12 2019
02 12 2019
Historique:
received:
12
02
2019
revised:
21
07
2019
accepted:
17
09
2019
pubmed:
23
10
2019
medline:
2
6
2020
entrez:
23
10
2019
Statut:
ppublish
Résumé
During mitosis, the centrosome expands its capacity to nucleate microtubules. Understanding the mechanisms of centrosomal microtubule nucleation is, however, constrained by a lack of knowledge of the amount of soluble and polymeric tubulin at mitotic centrosomes. Here we combined light microscopy and serial-section electron tomography to measure the amount of dimeric and polymeric tubulin at mitotic centrosomes in early
Identifiants
pubmed: 31636117
pii: jcb.201902069
doi: 10.1083/jcb.201902069
pmc: PMC6891098
doi:
Substances chimiques
Polymers
0
Tubulin
0
Nocodazole
SH1WY3R615
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3977-3985Subventions
Organisme : NIDA NIH HHS
ID : K05 DA000074
Pays : United States
Informations de copyright
© 2019 Baumgart et al.
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