ER-shaping atlastin proteins act as central hubs to promote flavivirus replication and virion assembly.
A549 Cells
ADP-Ribosylation Factors
Animals
Chlorocebus aethiops
Dengue Virus
/ genetics
Endoplasmic Reticulum
/ metabolism
Flavivirus
/ genetics
GTP Phosphohydrolases
/ genetics
Gene Knockout Techniques
HEK293 Cells
HeLa Cells
Humans
Membrane Proteins
/ metabolism
Vero Cells
Viral Proteins
Virion
/ metabolism
Virus Assembly
Virus Replication
/ physiology
Zika Virus
/ genetics
Journal
Nature microbiology
ISSN: 2058-5276
Titre abrégé: Nat Microbiol
Pays: England
ID NLM: 101674869
Informations de publication
Date de publication:
12 2019
12 2019
Historique:
received:
16
02
2019
accepted:
10
09
2019
pubmed:
23
10
2019
medline:
1
7
2020
entrez:
23
10
2019
Statut:
ppublish
Résumé
Flaviviruses, including dengue virus and Zika virus, extensively remodel the cellular endomembrane network to generate replication organelles that promote viral genome replication and virus production. However, it remains unclear how these membranes and associated cellular proteins act during the virus cycle. Here, we show that atlastins (ATLs), a subset of ER resident proteins involved in neurodegenerative diseases, have dichotomous effects on flaviviruses-with ATL2 depletion leading to replication organelle defects, and ATL3 depletion to changes in virus production pathways. We characterized non-conserved functional domains in ATL paralogues and show that the ATL interactome is profoundly reprogrammed following dengue virus infection. Screen analysis confirmed non-redundant ATL functions and identified a specific role for ATL3, and its interactor ARF4, in vesicle trafficking and virion maturation. Our data identify ATLs as central hubs targeted by flaviviruses to establish their replication organelle and to achieve efficient virion maturation and secretion.
Identifiants
pubmed: 31636417
doi: 10.1038/s41564-019-0586-3
pii: 10.1038/s41564-019-0586-3
pmc: PMC6881184
mid: EMS84351
doi:
Substances chimiques
Membrane Proteins
0
Viral Proteins
0
ATL2 protein, human
EC 3.6.1.-
GTP Phosphohydrolases
EC 3.6.1.-
ATL3 protein, human
EC 3.6.5.-
ADP-Ribosylation Factors
EC 3.6.5.2
ARF4 protein, human
EC 3.6.5.2
ARF5 protein, human
EC 3.6.5.2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2416-2429Subventions
Organisme : European Research Council
ID : 311339
Pays : International
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