Analytical Differences in Intraoperative Parathyroid Hormone Assays.


Journal

The journal of applied laboratory medicine
ISSN: 2576-9456
Titre abrégé: J Appl Lab Med
Pays: England
ID NLM: 101693884

Informations de publication

Date de publication:
03 2019
Historique:
received: 10 04 2018
accepted: 07 12 2018
entrez: 23 10 2019
pubmed: 23 10 2019
medline: 18 9 2020
Statut: ppublish

Résumé

We compared the rates of intraoperative parathyroid hormone (PTH) decline using the Siemens Immulite Serial blood samples from 95 patients undergoing parathyroidectomy were collected and measured using the 2 immunoassays. Specimens from the first 15 patients were measured simultaneously in the OR and CCL and used for the TAT study. In addition to 2 baseline samples, specimens were collected at 5, 10, and 15 min (for some patients, >15 min) after parathyroidectomy. In the TAT study, a significant difference was observed (OR median 20 min vs CCL median 27 min; There was a slightly longer TAT in the CCL compared with running the assay directly within the OR (median difference of approximately 7 min). For a majority of the patients, both methods showed equivalent rates of PTH decline; however, for approximately 20% of the patients, there was a slower rate of PTH decline using the Roche assay.

Sections du résumé

BACKGROUND
We compared the rates of intraoperative parathyroid hormone (PTH) decline using the Siemens Immulite
METHODS
Serial blood samples from 95 patients undergoing parathyroidectomy were collected and measured using the 2 immunoassays. Specimens from the first 15 patients were measured simultaneously in the OR and CCL and used for the TAT study. In addition to 2 baseline samples, specimens were collected at 5, 10, and 15 min (for some patients, >15 min) after parathyroidectomy.
RESULTS
In the TAT study, a significant difference was observed (OR median 20 min vs CCL median 27 min;
CONCLUSIONS
There was a slightly longer TAT in the CCL compared with running the assay directly within the OR (median difference of approximately 7 min). For a majority of the patients, both methods showed equivalent rates of PTH decline; however, for approximately 20% of the patients, there was a slower rate of PTH decline using the Roche assay.

Identifiants

pubmed: 31639754
pii: jalm.2018.026815
doi: 10.1373/jalm.2018.026815
doi:

Substances chimiques

Parathyroid Hormone 0

Types de publication

Comparative Study Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

788-798

Informations de copyright

© 2018 American Association for Clinical Chemistry.

Auteurs

Edward K Y Leung (EKY)

Department of Pathology, Pritzker School of Medicine, The University of Chicago, Chicago, IL; edleung@chla.usc.edu.

Christine C Lee (CC)

Department of Pathology, Pritzker School of Medicine, The University of Chicago, Chicago, IL.

Peter Angelos (P)

Endocrine Surgery Research Program, Department of Surgery, Section of Endocrine Surgery, The University of Chicago Medical Center, Chicago, IL.

Edwin L Kaplan (EL)

Endocrine Surgery Research Program, Department of Surgery, Section of Endocrine Surgery, The University of Chicago Medical Center, Chicago, IL.

Raymon H Grogan (RH)

Endocrine Surgery Research Program, Department of Surgery, Section of Endocrine Surgery, The University of Chicago Medical Center, Chicago, IL.

David A Sarracino (DA)

BRIMS (Biomarker Research Initiative in MS), Thermo Fisher Scientific, Cambridge, MA.

Bryan Krastins (B)

Orb Health, Phoenix, AZ.

Mary F Lopez (MF)

InformaDX, Bedford, MA.

Theodore Karrison (T)

Department of Public Health Sciences, Biostatistics Laboratory, The University of Chicago, Chicago, IL.

Kiang-Teck J Yeo (KJ)

Department of Pathology, Pritzker School of Medicine, The University of Chicago, Chicago, IL.

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