Clinical characteristics and molecular epidemiology of children with meningitis in Tehran, Iran: a prospective study.

Children diagnosis genotype meningitis molecular epidemiology

Journal

New microbes and new infections
ISSN: 2052-2975
Titre abrégé: New Microbes New Infect
Pays: England
ID NLM: 101624750

Informations de publication

Date de publication:
Nov 2019
Historique:
received: 08 03 2019
revised: 18 07 2019
accepted: 20 08 2019
entrez: 24 10 2019
pubmed: 24 10 2019
medline: 24 10 2019
Statut: epublish

Résumé

The molecular epidemiology of meningitis in children is unclear in Iran, and data are scarce. We aimed to characterize its clinical and paraclinical features as well as to determine the distribution of genotype/capsular types of common bacterial meningitis agents in children in Iran. All children suspected to have meningitis aged 4 days to 15 years were enrolled onto a prospective cross-sectional study from January 2015 to September 2017. Diagnostic values of clinical features, cerebrospinal fluid and serum parameters were evaluated independently and in combination with each other by multivariate logistic regression to develop a diagnostic rule. Genotype/capsular types of all the isolates were determined by targeting serotype-specific genes with uniplex or multiplex PCR. Among 119 patients suspected of having meningitis, 43 had bacterial meningitis, 19 aseptic and one tuberculous; and there were 56 nonmeningitis cases (NMC). Presentation of four features at the same time-cerebrospinal fluid white blood cell count, protein, polymorphonuclear leukocytes and serum C-reactive protein-revealed 100% sensitivity and 86.4% specificity for diagnosis of bacterial meningitis.

Identifiants

pubmed: 31641511
doi: 10.1016/j.nmni.2019.100594
pii: S2052-2975(19)30091-5
pii: 100594
pmc: PMC6796727
doi:

Types de publication

Journal Article

Langues

eng

Pagination

100594

Informations de copyright

© 2019 Published by Elsevier Ltd.

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Auteurs

A Pormohammad (A)

Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

S Lashkarbolouki (S)

Department of Biology, Tehran North Branch, Islamic Azad University, Tehran, Iran.

T Azimi (T)

Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Students Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran.

P Gholizadeh (P)

Department of Medical Microbiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.

N Bostanghadiri (N)

Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

H Safari (H)

Health Promotion Research Center, school of public health, Iran University of Medical Sciences, Tehran, Iran.

S Armin (S)

Pediatric Infections Research Center, Research Institute for Children Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

N Mohtavinejad (N)

Department of Radio Pharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.

F Fallah (F)

Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Classifications MeSH