Live imaging of contracting muscles with wide-field second harmonic generation microscopy using a high power laser.


Journal

Biomedical optics express
ISSN: 2156-7085
Titre abrégé: Biomed Opt Express
Pays: United States
ID NLM: 101540630

Informations de publication

Date de publication:
01 Oct 2019
Historique:
received: 18 06 2019
revised: 29 08 2019
accepted: 29 08 2019
entrez: 25 10 2019
pubmed: 28 10 2019
medline: 28 10 2019
Statut: epublish

Résumé

Wide-field second harmonic generation (SHG) microscopy was developed using a high-power (> 4 W) and high-repetition-rate (MHz range) laser oscillator to achieve fast SHG imaging over a large area (400 µm × 400 µm). The microscope was used for high spatial resolution imaging of contracting muscles in live

Identifiants

pubmed: 31646035
doi: 10.1364/BOE.10.005130
pii: 369870
pmc: PMC6788594
doi:

Types de publication

Journal Article

Langues

eng

Pagination

5130-5135

Informations de copyright

© 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.

Déclaration de conflit d'intérêts

The authors declare that there are no conflicts of interest related to this article.

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Auteurs

Haitao Zhao (H)

WDI Device Inc., 135 West Beaver Creek Road Unit 2, Richmond Hill, ON, L4B 1L2, Canada.
Department of Chemical and Physical Sciences, University of Toronto Mississauga, 3359 Mississauga Road, Mississauga, ON L5L 1C6, Canada.
Department of Physics, University of Toronto, 60 St. George Street, Toronto, ON M5S 1A7, Canada.
Co-first authors with equal contribution.

Richard Cisek (R)

Department of Chemistry, Saint Mary's University, 923 Robie Street, Halifax, NS B3H 3C3, Canada.
Co-first authors with equal contribution.

Abiramy Karunendiran (A)

Department of Biology, University of Toronto Mississauga, 3359 Mississauga Road, Mississauga, ON L5L 1C6, Canada.
Department of Cell and Systems Biology, University of Toronto, 25 Harbord Street, Toronto, ON, M5S 3G5, Canada.

Danielle Tokarz (D)

Department of Chemistry, Saint Mary's University, 923 Robie Street, Halifax, NS B3H 3C3, Canada.

Bryan A Stewart (BA)

Department of Biology, University of Toronto Mississauga, 3359 Mississauga Road, Mississauga, ON L5L 1C6, Canada.
Department of Cell and Systems Biology, University of Toronto, 25 Harbord Street, Toronto, ON, M5S 3G5, Canada.

Virginijus Barzda (V)

Department of Chemical and Physical Sciences, University of Toronto Mississauga, 3359 Mississauga Road, Mississauga, ON L5L 1C6, Canada.
Department of Physics, University of Toronto, 60 St. George Street, Toronto, ON M5S 1A7, Canada.

Classifications MeSH