Stimulated Raman histology: one to one comparison with standard hematoxylin and eosin staining.

We present for the first time one-to-one correspondence between standard hematoxylin/eosin (H&E) stained tissue sections and stimulated Raman histology (SRH) - a label-free technique in which stimulated Raman scattering (SRS) and second harmonic generation (SHG) are combined to generate virtual H&E images. Experiments were performed on both human thin cryogenic slides from the gastrointestinal tract (GI) and thick freshly excised biopsies from endoscopic surgery. Results on cryogenic slides evidenced an excellent agreement between SRH and H&E images while the ones on biopsies established the relevance of SRH for rapid intraoperative histology to assist in surgical decision making.

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© 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.

Déclaration de conflit d'intérêts

The authors declare that there are no conflicts of interest related to this article. All tissue sections were collected in the context of Institut Paoli-Calmettes approved protocol from patients who provided informed consent. Tissues in excess of what was needed for diagnosis were eligible for imaging. All methods were performed in accordance with the European Union guidelines and regulations Commission Directive 2006/17/EC of 8 February 2006 as regards technical requirements for the donation, procurement and testing of human tissues and cells.