Ratiometric widefield imaging with spectrally balanced detection.
Journal
Biomedical optics express
ISSN: 2156-7085
Titre abrégé: Biomed Opt Express
Pays: United States
ID NLM: 101540630
Informations de publication
Date de publication:
01 Oct 2019
01 Oct 2019
Historique:
received:
24
06
2019
revised:
12
08
2019
accepted:
29
08
2019
entrez:
25
10
2019
pubmed:
28
10
2019
medline:
28
10
2019
Statut:
epublish
Résumé
Ratiometric imaging is an invaluable tool for quantitative microscopy, allowing for robust detection of FRET, anisotropy, and spectral shifts of nano-scale optical probes in response to local physical and chemical variations such as local pH, ion composition, and electric potential. In this paper, we propose and demonstrate a scheme for widefield ratiometric imaging that allows for continuous tuning of the cutoff wavelength between its two spectral channels. This scheme is based on angle-tuning the image splitting dichroic beamsplitter, similar to previous works on tunable interference filters. This configuration allows for ratiometric imaging of spectrally heterogeneous samples, which require spectral tunability of the detection path in order to achieve good spectrally balanced ratiometric detection.
Identifiants
pubmed: 31646053
doi: 10.1364/BOE.10.005385
pii: 370784
pmc: PMC6788590
doi:
Types de publication
Journal Article
Langues
eng
Pagination
5385-5394Informations de copyright
© 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.
Déclaration de conflit d'intérêts
The authors declare that there are no conflicts of interest related to this article.
Références
Adv Biol Med Phys. 1957;5:147-209
pubmed: 13520431
Biophys J. 1999 Nov;77(5):2864-70
pubmed: 10545384
J Physiol. 2005 Sep 1;567(Pt 2):523-43
pubmed: 15946962
Cytometry. 1993 Nov;14(8):916-21
pubmed: 8287734
ACS Photonics. 2018 Jul 18;5(7):2860-2867
pubmed: 30042952
Phys Chem Chem Phys. 2015 Nov 7;17(41):27862-72
pubmed: 26439729
Biochemistry. 1989 May 30;28(11):4536-9
pubmed: 2765500
IEEE Trans Biomed Eng. 2011 Jul;58(7):2120-6
pubmed: 21536528
Ann Biomed Eng. 2006 Apr;34(4):642-52
pubmed: 16547608
Biophys J. 1990 Jun;57(6):1179-86
pubmed: 2393703
Am J Physiol. 1999 Mar;276(3):C747-57
pubmed: 10070003
J Biomed Opt. 2014 Jan;19(1):011017
pubmed: 24077519
J Vis Exp. 2009 Nov 19;(33):null
pubmed: 19927116
Nat Protoc. 2011 Dec 08;7(1):24-35
pubmed: 22157973
Biophys J. 2001 Aug;81(2):1163-70
pubmed: 11463657
J Biol Chem. 1985 Mar 25;260(6):3440-50
pubmed: 3838314
Chem Rev. 2006 May;106(5):1785-813
pubmed: 16683755
ACS Nano. 2012 Nov 27;6(11):10013-23
pubmed: 23075136
Neuron. 1992 Sep;9(3):393-403
pubmed: 1524823
Curr Protoc Cytom. 2011 Apr;Chapter 2:Unit 2.4
pubmed: 21455966
J Gen Physiol. 2013 Mar;141(3):371-87
pubmed: 23440278