Multimeric Ionotropic Purinoceptor Detection by Protein Cross-Linking.
Cross-linking
Heteromer
Homomer
P2X
Quaternary structure
Trimer
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
25
10
2019
pubmed:
28
10
2019
medline:
18
4
2020
Statut:
ppublish
Résumé
P2X receptor subunits (P2X1 to P2X7) assemble to form trimeric homomers or heteromers. Here, we describe the use of protein cross-linking to study the composition of P2X receptor complexes. This simple protocol is useful for determining the stoichiometry of P2X heteromeric receptors as well as for assessing the effect of point mutation, truncation, or concatenation on the quaternary architecture of these receptors.
Identifiants
pubmed: 31646486
doi: 10.1007/978-1-4939-9717-6_10
doi:
Substances chimiques
Cross-Linking Reagents
0
Protein Subunits
0
Receptors, Purinergic
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM