Comparison of pre-analytical conditions for quantification of serotonin in platelet-poor plasma.
5-HT
5-Hydroxytryptamine
Plasma
Platelet-poor plasma
Pre-analytical procedure
Serotonin
Journal
Practical laboratory medicine
ISSN: 2352-5517
Titre abrégé: Pract Lab Med
Pays: Netherlands
ID NLM: 101690848
Informations de publication
Date de publication:
Nov 2019
Nov 2019
Historique:
received:
24
06
2019
revised:
08
08
2019
accepted:
17
09
2019
entrez:
26
10
2019
pubmed:
28
10
2019
medline:
28
10
2019
Statut:
epublish
Résumé
Reported concentrations of serotonin in platelet-poor plasma (PPP) in healthy subjects vary widely due to different pre-analytical procedures. To examine how different pre-analytical conditions affect the measured concentration of serotonin in PPP. Six pre-analytical protocols were compared for preparation of PPP from EDTA whole blood for quantification of serotonin from nine healthy individuals. Three combinations of centrifugation with a mild centrifugation of gel-free EDTA tubes followed by a stronger centrifugation were compared to single-stage centrifugation of EDTA tubes with separator gel and heat shock treatment of blood prior to centrifugation. All samples were analysed using the same enzyme linked immunosorbent assay (ELISA) method. Findings show that two consecutive centrifugations; first a mild centrifugation at 100 or 200× Two successive centrifugations to produce PPP for serotonin analysis; first a mild centrifugation to avoid mechanical stress on the platelets, and next a stronger centrifugation to remove platelets, is superior to the use of gel tubes and heat shock treatment.
Sections du résumé
BACKGROUND
BACKGROUND
Reported concentrations of serotonin in platelet-poor plasma (PPP) in healthy subjects vary widely due to different pre-analytical procedures.
AIM
OBJECTIVE
To examine how different pre-analytical conditions affect the measured concentration of serotonin in PPP.
METHOD
METHODS
Six pre-analytical protocols were compared for preparation of PPP from EDTA whole blood for quantification of serotonin from nine healthy individuals. Three combinations of centrifugation with a mild centrifugation of gel-free EDTA tubes followed by a stronger centrifugation were compared to single-stage centrifugation of EDTA tubes with separator gel and heat shock treatment of blood prior to centrifugation. All samples were analysed using the same enzyme linked immunosorbent assay (ELISA) method.
RESULTS
RESULTS
Findings show that two consecutive centrifugations; first a mild centrifugation at 100 or 200×
CONCLUSION
CONCLUSIONS
Two successive centrifugations to produce PPP for serotonin analysis; first a mild centrifugation to avoid mechanical stress on the platelets, and next a stronger centrifugation to remove platelets, is superior to the use of gel tubes and heat shock treatment.
Identifiants
pubmed: 31649988
doi: 10.1016/j.plabm.2019.e00136
pii: S2352-5517(19)30066-6
pii: e00136
pmc: PMC6804510
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e00136Informations de copyright
© 2019 The Authors.
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