Molecular surveillance of hepatitis C virus genotypes identifies the emergence of a genotype 4d lineage among men in Quebec, 2001-2017.
G4d
HCV
MSM
cluster
genotype
men who have sex with men
molecular epidemiology
phylogenetic analyses
surveillance
Journal
Canada communicable disease report = Releve des maladies transmissibles au Canada
ISSN: 1188-4169
Titre abrégé: Can Commun Dis Rep
Pays: Canada
ID NLM: 9303729
Informations de publication
Date de publication:
05 Sep 2019
05 Sep 2019
Historique:
entrez:
26
10
2019
pubmed:
28
10
2019
medline:
28
10
2019
Statut:
epublish
Résumé
Molecular phylogenetics are generally used to confirm hepatitis C virus (HCV) transmission events. In addition, the Laboratoire de santé publique du Québec (LSPQ) has been using molecular phylogenetics for surveillance of HCV genotyping since November 2001. To describe the emergence of a specific lineage of HCV genotype 4d (G4d) and its characteristics using molecular phylogenetics as a surveillance tool for identifying HCV strain clustering. The LSPQ prospectively applied Sanger sequencing and phylogenetic analysis to determine the HCV genotype on samples collected from November 2001 to December 2017. When a major G4d cluster was identified, demographic information, HIV-infection status and syphilis test results were analyzed. Phylogenetic analyses performed on approximately 22,000 cases identified 122 G4d cases. One major G4d cluster composed of 37 cases was singled out. Two cases were identified in 2010, 10 from 2011-2014 and 25 from 2015-2017. Cases in the cluster were concentrated in two urban health regions. Compared to the other G4d cases, cluster cases were all male ( Molecular phylogenetics enabled the identification and surveillance of ongoing transmission of a specific HCV G4d lineage in HIV-positive and HIV-negative men in Quebec and its cross-continental spread. This information can orient intervention strategies to avoid transmission of HCV in MSM.
Sections du résumé
BACKGROUND
BACKGROUND
Molecular phylogenetics are generally used to confirm hepatitis C virus (HCV) transmission events. In addition, the Laboratoire de santé publique du Québec (LSPQ) has been using molecular phylogenetics for surveillance of HCV genotyping since November 2001.
OBJECTIVES
OBJECTIVE
To describe the emergence of a specific lineage of HCV genotype 4d (G4d) and its characteristics using molecular phylogenetics as a surveillance tool for identifying HCV strain clustering.
METHODS
METHODS
The LSPQ prospectively applied Sanger sequencing and phylogenetic analysis to determine the HCV genotype on samples collected from November 2001 to December 2017. When a major G4d cluster was identified, demographic information, HIV-infection status and syphilis test results were analyzed.
RESULTS
RESULTS
Phylogenetic analyses performed on approximately 22,000 cases identified 122 G4d cases. One major G4d cluster composed of 37 cases was singled out. Two cases were identified in 2010, 10 from 2011-2014 and 25 from 2015-2017. Cases in the cluster were concentrated in two urban health regions. Compared to the other G4d cases, cluster cases were all male (
CONCLUSION
CONCLUSIONS
Molecular phylogenetics enabled the identification and surveillance of ongoing transmission of a specific HCV G4d lineage in HIV-positive and HIV-negative men in Quebec and its cross-continental spread. This information can orient intervention strategies to avoid transmission of HCV in MSM.
Identifiants
pubmed: 31650986
doi: 10.14745/ccdr.v45i09a02
pii: 450902
pmc: PMC6781953
doi:
Types de publication
Journal Article
Langues
eng
Pagination
230-237Déclaration de conflit d'intérêts
Conflict of interest: None.
Références
BMC Infect Dis. 2010 Aug 27;10:257
pubmed: 20799943
J Clin Virol. 2017 Jul;92:42-47
pubmed: 28521213
AIDS. 2006 Jan 9;20(2):233-40
pubmed: 16511416
PLoS One. 2011;6(12):e29322
pubmed: 22216248
AIDS. 2015 Nov;29(17):2335-45
pubmed: 26258525
Can J Gastroenterol Hepatol. 2014 May;28(5):243-50
pubmed: 24839620
Emerg Infect Dis. 2015 May;21(5):765-74
pubmed: 25897788
BMC Infect Dis. 2017 Mar 2;17(1):185
pubmed: 28253838
Can Commun Dis Rep. 2014 Dec 18;40(19):429-436
pubmed: 29769874
Hepatology. 2004 Jan;39(1):90-6
pubmed: 14752827
Lancet. 1995 May 13;345(8959):1211-3
pubmed: 7739308
J Clin Microbiol. 2007 Apr;45(4):1102-12
pubmed: 17287328
PLoS One. 2017 Oct 9;12(10):e0185866
pubmed: 29016621
Clin Infect Dis. 2018 Aug 31;67(6):845-853
pubmed: 29767683
Am J Epidemiol. 2004 Apr 1;159(7):702-6
pubmed: 15033648
Antivir Ther. 2012;17(7 Pt B):1465-70
pubmed: 23321496
Can J Infect Dis Med Microbiol. 2015 Jan-Feb;26(1):17-22
pubmed: 25798149
Open Forum Infect Dis. 2015 Aug 06;2(3):ofv115
pubmed: 26634219
AIDS. 2017 Jul 17;31(11):1603-1610
pubmed: 28657964
Genome Biol. 2014 Nov 18;15(11):538
pubmed: 25418119
Gastroenterology. 2009 May;136(5):1609-17
pubmed: 19422083
PLoS One. 2012;7(10):e47335
pubmed: 23110068
PLoS One. 2015 Jul 20;10(7):e0131437
pubmed: 26192190
AIDS. 2017 Sep 24;31(15):2147-2158
pubmed: 28692530
Hepatology. 2014 Nov;60(5):1571-1580
pubmed: 25042607
Infect Genet Evol. 2015 Jul;33:101-9
pubmed: 25917496
PLoS One. 2018 Jan 2;13(1):e0190340
pubmed: 29293630