Mapping Keratoconus Molecular Substrates by Multiplexed High-Resolution Proteomics of Unpooled Corneas.
Biological Transport
Biomarkers
Case-Control Studies
Chromatography, Liquid
Cornea
/ metabolism
Disease Susceptibility
Extracellular Matrix
Keratoconus
/ etiology
Mass Spectrometry
Nonsense Mediated mRNA Decay
Proteasome Endopeptidase Complex
/ metabolism
Proteome
Proteomics
/ methods
Ubiquitin
/ metabolism
Workflow
ER stress
complement
cornea
keratoconus
proteasomal degradation
proteomics
Journal
Omics : a journal of integrative biology
ISSN: 1557-8100
Titre abrégé: OMICS
Pays: United States
ID NLM: 101131135
Informations de publication
Date de publication:
11 2019
11 2019
Historique:
pubmed:
28
10
2019
medline:
14
5
2020
entrez:
26
10
2019
Statut:
ppublish
Résumé
Keratoconus (KCN) is a leading cause for cornea grafting worldwide. Keratoconus is a multifactorial disease that causes progressive thinning of the cornea and whose etiology is poorly understood. Several studies have used proteomics on patient tear fluids to identify potential biomarkers. However, proteome of the cornea itself has not been investigated fully. We report here new findings from a case-control study using multiplexed mass spectrometry (MS) on individual (unpooled) corneas to gain deeper insights into proteins and biomarkers relevant to keratoconus. We employed a high-pressure approach to extract total protein from individual corneas from five cases and five controls, followed by trypsin digestion and tandem mass tag (TMT) labeling. The MS-derived data were searched using the Human NCBI RefSeq protein database v92, with peptides and proteins filtered at 1% false discovery rate. A total of 3132 proteins were detected, of which 627 were altered significantly (
Identifiants
pubmed: 31651220
doi: 10.1089/omi.2019.0143
pmc: PMC6857467
doi:
Substances chimiques
Biomarkers
0
Proteome
0
Ubiquitin
0
Proteasome Endopeptidase Complex
EC 3.4.25.1
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
583-597Subventions
Organisme : NEI NIH HHS
ID : R01 EY026104
Pays : United States
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