The chromogranin A-derived antifungal peptide CGA-N9 induces apoptosis in Candida tropicalis.
Antifungal Agents
/ metabolism
Apoptosis
/ drug effects
Calcium
/ metabolism
Candida tropicalis
/ drug effects
Cell Membrane
/ drug effects
Chromatin
/ metabolism
Chromogranin A
/ chemistry
Cytochromes c
/ metabolism
Cytosol
/ metabolism
DNA Fragmentation
/ drug effects
DNA, Fungal
/ metabolism
Humans
Membrane Potential, Mitochondrial
/ drug effects
Mitochondria
/ metabolism
Peptide Fragments
/ metabolism
Reactive Oxygen Species
/ metabolism
Static Electricity
CGA-N9
antimicrobial peptide
cytochrome c
membrane potential
yeast apoptosis
Journal
The Biochemical journal
ISSN: 1470-8728
Titre abrégé: Biochem J
Pays: England
ID NLM: 2984726R
Informations de publication
Date de publication:
30 10 2019
30 10 2019
Historique:
received:
28
06
2019
revised:
04
10
2019
accepted:
14
10
2019
pubmed:
28
10
2019
medline:
14
7
2020
entrez:
26
10
2019
Statut:
ppublish
Résumé
CGA-N9, a peptide derived from human chromogranin A (CGA), was found to have antimicrobial activity in our previous investigation, but its mechanism of action remains unclear. Herein, the mechanism of action of CGA-N9 was investigated. We found that CGA-N9 induced the depolarization of the cell membrane and uptake of calcium ions into the cytosol and mitochondria. With the disruption of the mitochondrial membrane potential, the generation of intracellular reactive oxygen species (ROS) increased. Accordingly, we assessed apoptotic processes in Candida tropicalis cells post-treatment with CGA-N9 and found cytochrome c leakage, chromatin condensation and DNA degradation. The interaction of CGA-N9 with DNA in vitro showed that CGA-N9 did not degrade DNA but bound to DNA via an electrostatic interaction. In conclusion, CGA-N9 exhibits antifungal activity by inducing apoptosis in C. tropicalis.
Identifiants
pubmed: 31652303
pii: 220763
doi: 10.1042/BCJ20190483
pmc: PMC6824672
doi:
Substances chimiques
Antifungal Agents
0
CGA-N9 peptide
0
CHGA protein, human
0
Chromatin
0
Chromogranin A
0
DNA, Fungal
0
Peptide Fragments
0
Reactive Oxygen Species
0
Cytochromes c
9007-43-6
Calcium
SY7Q814VUP
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3069-3080Informations de copyright
© 2019 The Author(s).
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