Matrix Metalloproteinase-3 is Key Effector of TNF-α-Induced Collagen Degradation in Skin.


Journal

International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791

Informations de publication

Date de publication:
22 Oct 2019
Historique:
received: 10 10 2019
accepted: 17 10 2019
entrez: 27 10 2019
pubmed: 28 10 2019
medline: 4 3 2020
Statut: epublish

Résumé

Inflammatory processes in the skin augment collagen degradation due to the up-regulation of matrix metalloproteinases (MMPs). The aim of the present project was to study the specific impact of MMP-3 on collagen loss in skin and its interplay with the collagenase MMP-13 under inflammatory conditions mimicked by the addition of the pro-inflammatory cytokine tumor necrosis factor-α (TNF-α). Skin explants from MMP-3 knock-out (KO) mice or from transgenic (TG) mice overexpressing MMP-3 in the skin and their respective wild-type counterparts (WT and WTT) were incubated ex vivo for eight days. The rate of collagen degradation, measured by released hydroxyproline, was reduced (

Identifiants

pubmed: 31652545
pii: ijms20205234
doi: 10.3390/ijms20205234
pmc: PMC6829232
pii:
doi:

Substances chimiques

Dipeptides 0
Matrix Metalloproteinase Inhibitors 0
N-(2(R)-2-(hydroxamidocarbonylmethyl)-4-methylpentanoyl)-L-tryptophan methylamide 0
Tumor Necrosis Factor-alpha 0
Collagen 9007-34-5
Matrix Metalloproteinase 13 EC 3.4.24.-
Mmp13 protein, mouse EC 3.4.24.-
Matrix Metalloproteinase 3 EC 3.4.24.17
Mmp3 protein, mouse EC 3.4.24.17

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Versus Arthritis
ID : 21447
Pays : United Kingdom
Organisme : European Research Council
ID : 243195
Pays : International

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Auteurs

Ursula Mirastschijski (U)

Center for Biomolecular Interactions Bremen, Department of Biology and Biochemistry, University of Bremen, 28359 Bremen, Germany. mirastsc@uni-bremen.de.

Blaž Lupše (B)

Center for Biomolecular Interactions Bremen, Department of Biology and Biochemistry, University of Bremen, 28359 Bremen, Germany. blazpikalupse@gmail.com.

Kathrin Maedler (K)

Center for Biomolecular Interactions Bremen, Department of Biology and Biochemistry, University of Bremen, 28359 Bremen, Germany. kmaedler@uni-bremen.de.

Bhavishya Sarma (B)

Center for Biomolecular Interactions Bremen, Department of Biology and Biochemistry, University of Bremen, 28359 Bremen, Germany. bhavishyasarma@yahoo.co.in.

Arlo Radtke (A)

Faculty of Biology and Biochemistry, University of Bremen, 28359 Bremen, Germany. arlo.radtke@uni-bremen.de.

Gazanfer Belge (G)

Faculty of Biology and Biochemistry, University of Bremen, 28359 Bremen, Germany. belge@uni-bremen.de.

Martina Dorsch (M)

Institute for Laboratory Animal Science, Hannover Medical School, 30625 Hannover, Germany. Dorsch.Martina@mh-hannover.de.

Dirk Wedekind (D)

Institute for Laboratory Animal Science, Hannover Medical School, 30625 Hannover, Germany. Wedekind.Dirk@mh-hannover.de.

Lisa J McCawley (LJ)

Department of Biomedical Engineering, Vanderbilt University, Nashville, TN 37232-6840, USA. lisa.mccawley@Vanderbilt.edu.

Gabriele Boehm (G)

Department of General, Visceral and Oncologic Surgery, Klinikum Bremen-Mitte, 28177 Bremen, Germany. Gabriele.Boehm@gesundheitnord.de.

Ulrich Zier (U)

Center for Biomolecular Interactions Bremen, Department of Biology and Biochemistry, University of Bremen, 28359 Bremen, Germany. u.x.zier@gmail.com.

Kazuhiro Yamamoto (K)

Institute of Ageing and Chronic Disease, University of Liverpool, Liverpool L69 3BX, United Kingdom. kazuhiro.yamamoto@liverpool.ac.uk.

Sørge Kelm (S)

Center for Biomolecular Interactions Bremen, Department of Biology and Biochemistry, University of Bremen, 28359 Bremen, Germany. skelm@uni-bremen.de.

Magnus S Ågren (MS)

Digestive Disease Center and Copenhagen Wound Healing Center, Bispebjerg Hospital, University of Copenhagen, 2400 Copenhagen, Denmark. magnus.agren@mail.dk.

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Classifications MeSH