Autologous micrograft accelerates endogenous wound healing response through ERK-induced cell migration.
Animals
Cell Movement
/ genetics
Cells, Cultured
Extracellular Signal-Regulated MAP Kinases
/ metabolism
Female
Fibroblasts
/ metabolism
Gene Expression Profiling
Gene Regulatory Networks
Keratinocytes
/ cytology
MAP Kinase Signaling System
/ genetics
Matrix Metalloproteinases
/ metabolism
Mice, Inbred C57BL
Skin Transplantation
Solubility
Transcription, Genetic
Transplantation, Autologous
Wound Healing
/ genetics
Journal
Cell death and differentiation
ISSN: 1476-5403
Titre abrégé: Cell Death Differ
Pays: England
ID NLM: 9437445
Informations de publication
Date de publication:
05 2020
05 2020
Historique:
received:
08
02
2019
accepted:
02
10
2019
revised:
30
09
2019
pubmed:
28
10
2019
medline:
1
7
2021
entrez:
27
10
2019
Statut:
ppublish
Résumé
Defective cell migration causes delayed wound healing (WH) and chronic skin lesions. Autologous micrograft (AMG) therapies have recently emerged as a new effective and affordable treatment able to improve wound healing capacity. However, the precise molecular mechanism through which AMG exhibits its beneficial effects remains unrevealed. Herein we show that AMG improves skin re-epithelialization by accelerating the migration of fibroblasts and keratinocytes. More specifically, AMG-treated wounds showed improvement of indispensable events associated with successful wound healing such as granulation tissue formation, organized collagen content, and newly formed blood vessels. We demonstrate that AMG is enriched with a pool of WH-associated growth factors that may provide the starting signal for a faster endogenous wound healing response. This work links the increased cell migration rate to the activation of the extracellular signal-regulated kinase (ERK) signaling pathway, which is followed by an increase in matrix metalloproteinase expression and their extracellular enzymatic activity. Overall we reveal the AMG-mediated wound healing transcriptional signature and shed light on the AMG molecular mechanism supporting its potential to trigger a highly improved wound healing process. In this way, we present a framework for future improvements in AMG therapy for skin tissue regeneration applications.
Identifiants
pubmed: 31654035
doi: 10.1038/s41418-019-0433-3
pii: 10.1038/s41418-019-0433-3
pmc: PMC7206041
doi:
Substances chimiques
Extracellular Signal-Regulated MAP Kinases
EC 2.7.11.24
Matrix Metalloproteinases
EC 3.4.24.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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