Nanographenes: Ultrastable, Switchable, and Bright Probes for Super-Resolution Microscopy.
blinking
chromophores
fluorescence
nanographenes
super-resolution imaging
Journal
Angewandte Chemie (International ed. in English)
ISSN: 1521-3773
Titre abrégé: Angew Chem Int Ed Engl
Pays: Germany
ID NLM: 0370543
Informations de publication
Date de publication:
02 01 2020
02 01 2020
Historique:
received:
24
07
2019
pubmed:
28
10
2019
medline:
28
10
2019
entrez:
29
10
2019
Statut:
ppublish
Résumé
Super-resolution fluorescence microscopy has enabled important breakthroughs in biology and materials science. Implementations such as single-molecule localization microscopy (SMLM) and minimal emission fluxes (MINFLUX) microscopy in the localization mode exploit fluorophores that blink, i.e., switch on and off, stochastically. Here, we introduce nanographenes, namely large polycyclic aromatic hydrocarbons that can also be regarded as atomically precise graphene quantum dots, as a new class of fluorophores for super-resolution fluorescence microscopy. Nanographenes exhibit outstanding photophysical properties: intrinsic blinking even in air, excellent fluorescence recovery, and stability over several months. As a proof of concept for super-resolution applications, we use nanographenes in SMLM to generate 3D super-resolution images of silica nanocracks. Our findings open the door for the widespread application of nanographenes in super-resolution fluorescence microscopy.
Identifiants
pubmed: 31657497
doi: 10.1002/anie.201909220
pmc: PMC6972658
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
496-502Subventions
Organisme : Human Frontiers in Science Program
ID : RGP0045/2018
Pays : International
Informations de copyright
© 2019 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.
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