Dysregulation of Placental Functions and Immune Pathways in Complete Hydatidiform Moles.


Journal

International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791

Informations de publication

Date de publication:
10 Oct 2019
Historique:
received: 17 09 2019
revised: 28 09 2019
accepted: 30 09 2019
entrez: 30 10 2019
pubmed: 30 10 2019
medline: 1 4 2020
Statut: epublish

Résumé

Gene expression studies of molar pregnancy have been limited to a small number of candidate loci. We analyzed high-dimensional RNA and protein data to characterize molecular features of complete hydatidiform moles (CHMs) and corresponding pathologic pathways. CHMs and first trimester placentas were collected, histopathologically examined, then flash-frozen or paraffin-embedded. Frozen CHMs and control placentas were subjected to RNA-Seq, with resulting data and published placental RNA-Seq data subjected to bioinformatics analyses. Paraffin-embedded tissues from CHMs and control placentas were used for tissue microarray (TMA) construction, immunohistochemistry, and immunoscoring for galectin-14. Of the 14,022 protein-coding genes expressed in all samples, 3,729 were differentially expressed (DE) in CHMs, of which 72% were up-regulated. DE genes were enriched in placenta-specific genes (OR = 1.88,

Identifiants

pubmed: 31658584
pii: ijms20204999
doi: 10.3390/ijms20204999
pmc: PMC6829352
pii:
doi:

Substances chimiques

Cytokines 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Hungarian Scientific Research Fund
ID : K128262
Organisme : Hungarian Scientific Research Fund
ID : K124862
Organisme : Magyar Tudományos Akadémia
ID : Momentum LP2014-7/2014
Organisme : Magyar Tudományos Akadémia
ID : Medinprot Protein Science Research Synergy IV and Adhoc Program grants
Organisme : Magyar Tudományos Akadémia
ID : Premium_2019-436 grant
Organisme : Magyar Tudományos Akadémia
ID : Young Research Fellowships
Organisme : Hungarian National Research, Development and Innovation Office
ID : FIEK_16-1-2016-0005
Organisme : Hungarian Ministry for National Economy
ID : VEKOP-2.3.3-15-2017-0014
Organisme : Gynecologic Oncology Research Fund
ID : USC

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Auteurs

Jennifer R King (JR)

Department of Obstetrics and Gynecology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA. jennyrenaeking@gmail.com.

Melissa L Wilson (ML)

Department of Preventive Medicine, University of Southern California, Los Angeles, CA 90033, USA. melisslw@usc.edu.

Szabolcs Hetey (S)

Systems Biology of Reproduction Research Group, Institute of Enzymology, Research Centre for Natural Sciences, H-1117 Budapest, Hungary. heteysz@gmail.com.

Peter Kiraly (P)

Systems Biology of Reproduction Research Group, Institute of Enzymology, Research Centre for Natural Sciences, H-1117 Budapest, Hungary. peter0kiraly@gmail.com.

Koji Matsuo (K)

Department of Obstetrics and Gynecology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA. koji.matsuo@med.usc.edu.

Antonio V Castaneda (AV)

Department of Pathology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA. antonio.castaneda@osumc.edu.

Eszter Toth (E)

Systems Biology of Reproduction Research Group, Institute of Enzymology, Research Centre for Natural Sciences, H-1117 Budapest, Hungary. toth.eszter@ttk.hu.

Tibor Krenacs (T)

First Department of Pathology and Experimental Cancer Research, Semmelweis University, H-1085 Budapest, Hungary. krenacst@gmail.com.

Petronella Hupuczi (P)

Maternity Private Clinic of Obstetrics and Gynecology, H-1126 Budapest, Hungary. hupuczi.petronella@maternity.hu.

Paulette Mhawech-Fauceglia (P)

Department of Pathology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA. pfauceglia@hotmail.com.

Andrea Balogh (A)

Systems Biology of Reproduction Research Group, Institute of Enzymology, Research Centre for Natural Sciences, H-1117 Budapest, Hungary. balogh.andrea@ttk.hu.

Andras Szilagyi (A)

Systems Biology of Reproduction Research Group, Institute of Enzymology, Research Centre for Natural Sciences, H-1117 Budapest, Hungary. szilagyi.andras@ttk.hu.

Janos Matko (J)

Department of Immunology, Institute of Biology, Eotvos Lorand University, H-1117 Budapest, Hungary. matko@elte.hu.

Zoltan Papp (Z)

Maternity Private Clinic of Obstetrics and Gynecology, H-1126 Budapest, Hungary. pzorvosihetilap@maternity.hu.
Department of Obstetrics and Gynecology, Semmelweis University, H-1088 Budapest, Hungary. pzorvosihetilap@maternity.hu.

Lynda D Roman (LD)

Department of Obstetrics and Gynecology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA. lroman@usc.edu.

Victoria K Cortessis (VK)

Department of Obstetrics and Gynecology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA. victoria.cortessis@med.usc.edu.
Department of Preventive Medicine, University of Southern California, Los Angeles, CA 90033, USA. victoria.cortessis@med.usc.edu.

Nandor Gabor Than (NG)

Department of Obstetrics and Gynecology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA. than.gabor@ttk.hu.
Systems Biology of Reproduction Research Group, Institute of Enzymology, Research Centre for Natural Sciences, H-1117 Budapest, Hungary. than.gabor@ttk.hu.
First Department of Pathology and Experimental Cancer Research, Semmelweis University, H-1085 Budapest, Hungary. than.gabor@ttk.hu.
Maternity Private Clinic of Obstetrics and Gynecology, H-1126 Budapest, Hungary. than.gabor@ttk.hu.

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