Noninvasive measurement of cell/colony motion using image analysis methods to evaluate the proliferative capacity of oral keratinocytes as a tool for quality control in regenerative medicine.
Oral keratinocyte
cell/colony motion
image analysis
quality control
regenerative medicine
Journal
Journal of tissue engineering
ISSN: 2041-7314
Titre abrégé: J Tissue Eng
Pays: England
ID NLM: 101550131
Informations de publication
Date de publication:
Historique:
received:
11
06
2019
accepted:
19
09
2019
entrez:
31
10
2019
pubmed:
31
10
2019
medline:
31
10
2019
Statut:
epublish
Résumé
Image-based cell/colony analyses offer promising solutions to compensate for the lack of quality control (QC) tools for noninvasive monitoring of cultured cells, a regulatory challenge in regenerative medicine. Here, the feasibility of two image analysis algorithms, optical flow and normalised cross-correlation, to noninvasively measure cell/colony motion in human primary oral keratinocytes for screening the proliferative capacity of cells in the early phases of cell culture were examined. We applied our software to movies converted from 96 consecutive time-lapse phase-contrast images of an oral keratinocyte culture. After segmenting the growing colonies, two indices were calculated based on each algorithm. The correlation between each index of the colonies and their proliferative capacity was evaluated. The software was able to assess cell/colony motion noninvasively, and each index reflected the observed cell kinetics. A positive linear correlation was found between cell/colony motion and proliferative capacity, indicating that both algorithms are potential tools for QC.
Identifiants
pubmed: 31662840
doi: 10.1177/2041731419881528
pii: 10.1177_2041731419881528
pmc: PMC6794654
doi:
Types de publication
Journal Article
Langues
eng
Pagination
2041731419881528Informations de copyright
© The Author(s) 2019.
Déclaration de conflit d'intérêts
Declaration of conflicting interests: The author(s) declared no potential conflicts of interest with respect to the research, authorship and/or publication of this article.
Références
Sci Rep. 2017 May 15;7(1):1897
pubmed: 28507330
J Dent Res. 2015 Jan;94(1):78-84
pubmed: 25348542
Int J Biochem Cell Biol. 2017 Mar;84:89-95
pubmed: 28111333
PLoS One. 2013 Nov 27;8(11):e81266
pubmed: 24312283
N Engl J Med. 2004 Sep 16;351(12):1187-96
pubmed: 15371576
Trends Biotechnol. 2017 Oct;35(10):971-982
pubmed: 28711155
J Oral Maxillofac Surg. 1999 May;57(5):571-7; discussion 577-8
pubmed: 10319831
Int J Oral Maxillofac Surg. 2003 Apr;32(2):188-97
pubmed: 12729781
Dev Growth Differ. 2013 May;55(4):523-49
pubmed: 23560739
IEEE Trans Pattern Anal Mach Intell. 2019 May;41(5):1131-1143
pubmed: 29993926
New J Phys. 2013 Jul;15:null
pubmed: 24363603
Int J Oral Sci. 2013 Sep;5(3):121-9
pubmed: 23887128
J Orthop Translat. 2017 Mar 02;9:1-7
pubmed: 29662794
Gut. 2006 Dec;55(12):1704-10
pubmed: 16709659
Regen Ther. 2018 Jul 09;9:15-23
pubmed: 30525071
Biomaterials. 2014 Aug;35(25):6667-76
pubmed: 24854093
Nat Commun. 2018 Sep 10;9(1):3665
pubmed: 30202009
J Cell Biol. 2015 Apr 27;209(2):305-15
pubmed: 25897083
Curr Mol Med. 2014 Feb;14(2):291-307
pubmed: 24467201
Transplantation. 2000 Dec 15;70(11):1588-98
pubmed: 11152220
J Med Imaging (Bellingham). 2017 Oct;4(4):044003
pubmed: 29134187
PLoS One. 2013 Jul 31;8(7):e69574
pubmed: 23936049
Sci Rep. 2017 Oct 26;7(1):14135
pubmed: 29075007
J Biosci Bioeng. 2016 Feb;121(2):227-34
pubmed: 26183859
N Engl J Med. 2010 Jul 8;363(2):147-55
pubmed: 20573916
J Lab Autom. 2014 Oct;19(5):454-60
pubmed: 24888327
Biotechnol Prog. 2016 Jan-Feb;32(1):215-23
pubmed: 26560993
Cold Spring Harb Perspect Biol. 2017 Apr 3;9(4):null
pubmed: 28096261
Front Neuroinform. 2019 Mar 12;13:15
pubmed: 30914941
Trends Cell Biol. 2016 Feb;26(2):88-110
pubmed: 26481052
IEEE Trans Biomed Eng. 1991 Mar;38(3):280-6
pubmed: 2066142
EMBO Mol Med. 2018 Feb;10(2):139-150
pubmed: 29288165
Biotechniques. 2019 Mar;66(3):128-133
pubmed: 30869548
Nat Commun. 2017 Dec 19;8(1):2194
pubmed: 29259161
PLoS One. 2016 Feb 05;11(2):e0148642
pubmed: 26848582
Cells Tissues Organs. 2004;176(1-3):134-52
pubmed: 14745242
Cytotherapy. 2013 Dec;15(12):1527-40
pubmed: 23992827
Cells Tissues Organs. 2014;199(5-6):393-404
pubmed: 25720390
J Biomol Screen. 2016 Oct;21(9):887-96
pubmed: 27256155
Cytometry. 2000 Sep 1;41(1):19-30
pubmed: 10942892
Int J Mol Sci. 2018 Jun 07;19(6):null
pubmed: 29880745