Long-term protective efficacy with a BCG-prime ID93/GLA-SE boost regimen against the hyper-virulent Mycobacterium tuberculosis strain K in a mouse model.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
29 10 2019
Historique:
received: 14 06 2019
accepted: 04 10 2019
entrez: 31 10 2019
pubmed: 31 10 2019
medline: 3 11 2020
Statut: epublish

Résumé

Since ID93/GLA-SE was developed as a targeted BCG-prime booster vaccine, in the present study, we evaluated the protective efficacy of ID93/GLA-SE as a boost to a BCG-prime against the hypervirulent Mycobacterium tuberculosis (Mtb) K challenge to provide further information on the development and application of this vaccine candidate. Boosting BCG with the ID93/GLA-SE vaccine significantly reduced bacterial burden at 16 weeks post-challenge while the BCG vaccine alone did not confer significant protection against Mtb K. The pathological analysis of the lung from the challenged mice also showed the remarkably protective boosting effect of ID93/GLA-SE on BCG-immunised animals. Moreover, qualitative and quantitative analysis of the immune responses following ID93/GLA-SE-immunisation demonstrated that ID93/GLA-SE was able to elicit robust and sustained Th1-biased antigen-specific multifunctional CD4

Identifiants

pubmed: 31664157
doi: 10.1038/s41598-019-52146-0
pii: 10.1038/s41598-019-52146-0
pmc: PMC6820558
doi:

Substances chimiques

Antigens, Bacterial 0
BCG Vaccine 0
Glucosides 0
Lipid A 0
Tuberculosis Vaccines 0
glucopyranosyl lipid-A 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

15560

Subventions

Organisme : NIAID NIH HHS
ID : R01 AI125160
Pays : United States
Organisme : NIAID NIH HHS
ID : T32 AI007509
Pays : United States
Organisme : U.S. Department of Health & Human Services | NIH | National Institute of Allergy and Infectious Diseases (NIAID)
ID : R01AI125160
Pays : International

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Auteurs

Kee Woong Kwon (KW)

Department of Microbiology, Institute for Immunology and Immunological Disease, Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul, 03722, South Korea.

Ara Lee (A)

Department of Biochemistry, College of Life Science & Biotechnology, Yonsei University, Seoul, 03722, South Korea.

Sasha E Larsen (SE)

Infectious Disease Research Institute, 1616 Eastlake Ave E, Suite 400, Seattle, WA, 98102, USA.

Susan L Baldwin (SL)

Infectious Disease Research Institute, 1616 Eastlake Ave E, Suite 400, Seattle, WA, 98102, USA.

Rhea N Coler (RN)

Infectious Disease Research Institute, 1616 Eastlake Ave E, Suite 400, Seattle, WA, 98102, USA.
Department of Global Health, University of Washington, Seattle, USA.
PAI Life Sciences Inc., Seattle, USA.

Steven G Reed (SG)

Infectious Disease Research Institute, 1616 Eastlake Ave E, Suite 400, Seattle, WA, 98102, USA.

Sang-Nae Cho (SN)

Department of Microbiology, Institute for Immunology and Immunological Disease, Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul, 03722, South Korea.

Sang-Jun Ha (SJ)

Department of Biochemistry, College of Life Science & Biotechnology, Yonsei University, Seoul, 03722, South Korea.

Sung Jae Shin (SJ)

Department of Microbiology, Institute for Immunology and Immunological Disease, Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul, 03722, South Korea. sjshin@yuhs.ac.

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Classifications MeSH