Batf Pioneers the Reorganization of Chromatin in Developing Effector T Cells via Ets1-Dependent Recruitment of Ctcf.
Animals
Basic-Leucine Zipper Transcription Factors
/ metabolism
CCCTC-Binding Factor
/ metabolism
CD4-Positive T-Lymphocytes
/ metabolism
Chromatin
/ metabolism
Chromatin Assembly and Disassembly
Genome
Humans
Interferon Regulatory Factors
/ metabolism
Interleukins
/ metabolism
Lymphocyte Activation
/ immunology
Mice, Inbred C57BL
Protein Binding
Proto-Oncogene Protein c-ets-1
/ metabolism
Up-Regulation
/ genetics
Batf
CD4 T cell differentiation
Ctcf
Ets1
chromatin accessibility
chromatin looping
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
29 10 2019
29 10 2019
Historique:
received:
26
12
2018
revised:
28
06
2019
accepted:
20
09
2019
entrez:
31
10
2019
pubmed:
31
10
2019
medline:
26
9
2020
Statut:
ppublish
Résumé
The basic leucine zipper transcription factor activating transcription factor-like (Batf) contributes to transcriptional programming of multiple effector T cells and is required for T helper 17 (Th17) and T follicular helper (Tfh) cell development. Here, we examine mechanisms by which Batf initiates gene transcription in developing effector CD4 T cells. We find that, in addition to its pioneering function, Batf controls developmentally regulated recruitment of the architectural factor Ctcf to promote chromatin looping that is associated with lineage-specific gene transcription. The chromatin-organizing actions of Batf are largely dependent on Ets1, which appears to be indispensable for the Batf-dependent recruitment of Ctcf. Moreover, most of the Batf-dependent sites to which Ctcf is recruited lie outside of activating protein-1-interferon regulatory factor (Ap-1-Irf) composite elements (AICEs), indicating that direct involvement of Batf-Irf complexes is not required. These results identify a cooperative role for Batf, Ets1, and Ctcf in chromatin reorganization that underpins the transcriptional programming of effector T cells.
Identifiants
pubmed: 31665634
pii: S2211-1247(19)31261-6
doi: 10.1016/j.celrep.2019.09.064
pmc: PMC7182170
mid: NIHMS1542673
pii:
doi:
Substances chimiques
Basic-Leucine Zipper Transcription Factors
0
Batf protein, mouse
0
CCCTC-Binding Factor
0
Chromatin
0
Interferon Regulatory Factors
0
Interleukins
0
Proto-Oncogene Protein c-ets-1
0
interferon regulatory factor-4
0
interleukin-21
MKM3CA6LT1
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1203-1220.e7Subventions
Organisme : NIAID NIH HHS
ID : P30 AI027767
Pays : United States
Organisme : NIAID NIH HHS
ID : T32 AI007051
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA013148
Pays : United States
Organisme : NIAMS NIH HHS
ID : P30 AR048311
Pays : United States
Organisme : NIDDK NIH HHS
ID : F30 DK098911
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK115172
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI106352
Pays : United States
Organisme : NIAID NIH HHS
ID : UM1 AI106716
Pays : United States
Informations de copyright
Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.
Références
Blood. 2011 Jun 9;117(23):6198-201
pubmed: 21505191
Mol Cell. 2010 May 28;38(4):576-89
pubmed: 20513432
Nature. 2012 Oct 25;490(7421):543-6
pubmed: 22992523
Nat Commun. 2017 May 30;8:15628
pubmed: 28555647
Sci Rep. 2017 Dec 12;7(1):17402
pubmed: 29234109
Oncogene. 1995 Dec 7;11(11):2255-65
pubmed: 8570175
J Immunol. 2016 May 15;196(10):4378-89
pubmed: 27067007
Cell. 2012 Oct 12;151(2):289-303
pubmed: 23021777
Immunity. 1995 Aug;3(2):223-37
pubmed: 7648395
Nucleic Acids Res. 2016 May 5;44(8):3567-85
pubmed: 26673693
J Exp Med. 2007 Nov 26;204(12):2825-35
pubmed: 17967903
Proc Natl Acad Sci U S A. 2017 Nov 14;114(46):12111-12119
pubmed: 29078395
Immunity. 2006 Nov;25(5):717-29
pubmed: 17070076
Nucleic Acids Res. 2016 Jul 8;44(W1):W160-5
pubmed: 27079975
Mol Cell. 2017 Sep 21;67(6):1049-1058.e6
pubmed: 28938092
Genome Biol. 2009;10(3):R25
pubmed: 19261174
Immunity. 2015 May 19;42(5):877-89
pubmed: 25992861
Nat Rev Genet. 2014 Apr;15(4):234-46
pubmed: 24614316
Mol Cell. 2011 Jul 8;43(1):145-55
pubmed: 21726817
Immunity. 2003 Jul;19(1):145-53
pubmed: 12871646
Nat Rev Genet. 2016 Oct 14;17(11):661-678
pubmed: 27739532
Bioinformatics. 2009 Oct 15;25(20):2730-1
pubmed: 19654113
J Exp Med. 2016 May 2;213(5):687-96
pubmed: 27069114
Nat Immunol. 2007 Sep;8(9):967-74
pubmed: 17581537
J Immunol. 2009 Jan 15;182(2):999-1010
pubmed: 19124743
Nat Immunol. 2011 Jun;12(6):536-43
pubmed: 21572431
Mol Cell. 2014 Apr 10;54(1):56-66
pubmed: 24613343
J Immunol. 2007 Jan 1;178(1):345-51
pubmed: 17182572
BMC Genomics. 2013 Jun 28;14:428
pubmed: 23805837
Nature. 2010 Feb 11;463(7282):808-12
pubmed: 20072126
Nat Commun. 2017 Aug 21;8(1):301
pubmed: 28824171
Methods. 2012 Nov;58(3):192-203
pubmed: 22903059
Oncogene. 2000 Mar 30;19(14):1752-63
pubmed: 10777209
Nat Immunol. 2007 Sep;8(9):958-66
pubmed: 17676043
Immunity. 2009 Oct 16;31(4):551-64
pubmed: 19818655
EMBO J. 2016 Mar 1;35(5):515-35
pubmed: 26796577
Immunol Rev. 2013 Mar;252(1):24-40
pubmed: 23405893
Cell. 2013 Mar 14;152(6):1285-97
pubmed: 23498937
Nucleic Acids Res. 2014 Mar;42(5):3059-72
pubmed: 24371273
J Exp Med. 2010 May 10;207(5):933-42
pubmed: 20421391
Immunity. 2009 Jan 16;30(1):155-67
pubmed: 19144320
Genome Res. 2009 Sep;19(9):1639-45
pubmed: 19541911
Cell Death Differ. 2014 Jul;21(7):1050-60
pubmed: 24531538
Blood. 2014 Jul 31;124(5):737-49
pubmed: 24951427
Nat Biotechnol. 2014 Feb;32(2):171-178
pubmed: 24441470
Mol Cell Biol. 1999 Oct;19(10):7138-46
pubmed: 10490649
Nat Methods. 2013 Dec;10(12):1213-8
pubmed: 24097267
Science. 2012 Nov 16;338(6109):975-80
pubmed: 22983707
Genome Biol. 2008;9(9):R137
pubmed: 18798982
Nature. 2009 Jul 16;460(7253):405-9
pubmed: 19578362
Trends Genet. 2000 Sep;16(9):369-72
pubmed: 10973062
Oncotarget. 2015 Apr 10;6(10):7804-14
pubmed: 25762639
Nature. 2013 Apr 25;496(7446):461-8
pubmed: 23467089
Cell. 2012 Nov 21;151(5):981-93
pubmed: 23178119
Nucleic Acids Res. 2013 Nov;41(21):e201
pubmed: 24071585
Nat Immunol. 2006 Jul;7(7):773-82
pubmed: 16767092
Genes Dev. 2011 Nov 1;25(21):2227-41
pubmed: 22056668
J Immunol. 2016 Dec 1;197(11):4371-4381
pubmed: 27798167
J Biol Chem. 2001 Jan 26;276(4):2852-7
pubmed: 11053448
J Clin Invest. 2013 Nov;123(11):4641-53
pubmed: 24216482
Nat Rev Immunol. 2013 Jul;13(7):499-509
pubmed: 23787991
Immunity. 2004 Dec;21(6):865-76
pubmed: 15589174
BMC Genomics. 2015 Nov 25;16:1000
pubmed: 26608661
Genome Biol. 2015 Dec 01;16:259
pubmed: 26619908
Curr Opin Genet Dev. 2012 Oct;22(5):409-15
pubmed: 22922161
Nat Protoc. 2007;2(7):1722-33
pubmed: 17641637
PLoS One. 2008;3(10):e3408
pubmed: 18923650
Mol Cell. 2017 Mar 16;65(6):1068-1080.e5
pubmed: 28262505
Immunity. 2010 May 28;32(5):605-15
pubmed: 20493732
Nat Immunol. 2017 Apr;18(4):412-421
pubmed: 28166218
Nat Immunol. 2012 Dec;13(12):1196-204
pubmed: 23064439