A novel method for measuring diet-induced thermogenesis in mice.
Energy expenditure
Measurement of diet-induced thermogenesis in mice
Mice
Physical activity
Thermic effect of food
Journal
MethodsX
ISSN: 2215-0161
Titre abrégé: MethodsX
Pays: Netherlands
ID NLM: 101639829
Informations de publication
Date de publication:
2019
2019
Historique:
received:
11
03
2019
accepted:
23
08
2019
entrez:
1
11
2019
pubmed:
2
11
2019
medline:
2
11
2019
Statut:
epublish
Résumé
Diet-induced thermogenesis (DIT) refers to energy expenditure (EE) related to food consumption. Enhancing DIT can lead to weight loss. Factors that increase DIT are expected to lower body mass index and body fat mass. Although various methods have been developed for measuring DIT in humans, there is currently no method available for calculating absolute DIT values in mice. Therefore, we attempted to measure DIT in mice by applying the method more commonly used for humans. Mouse energy metabolism was first measured under fasting conditions; EE was plotted against the square root of the activity count, and a linear regression equation was fit to the data. Then, energy metabolism was measured in mice that were allowed to feed ad libitum, and EE was plotted in the same way. We calculated the DIT by subtracting the predicted EE value from the fed EE value for the same activity count. The methodology for measuring DIT in mice may be helpful for researching ways of combatting obesity by increasing DIT. •The methodology for measuring absolute DIT values in mice is developed.•For mice, the proportion of DIT compared with calorie intake and EE are 12.3% and 21.7%, respectively.
Identifiants
pubmed: 31667091
doi: 10.1016/j.mex.2019.08.016
pii: S2215-0161(19)30221-3
pmc: PMC6812409
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1950-1956Informations de copyright
© 2019 The Authors.
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