Immunological characterisation of truncated lipooligosaccharide-outer membrane protein based conjugate vaccine against Moraxella catarrhalis and nontypeable Haemophilus influenzae.
Animals
Antigens, Bacterial
/ immunology
Bacterial Outer Membrane Proteins
/ immunology
Bacterial Vaccines
/ administration & dosage
Female
Haemophilus Infections
/ immunology
Haemophilus Vaccines
/ administration & dosage
Haemophilus influenzae
/ immunology
Immunoglobulin G
/ immunology
Lipopolysaccharides
/ immunology
Mice
Mice, Inbred BALB C
Moraxella catarrhalis
/ immunology
Moraxellaceae Infections
/ immunology
Vaccination
Vaccines, Conjugate
/ immunology
Conjugate vaccine
Conserved antigens
Lipooligosaccharide mutant
Moraxella catarrhalis
Nontypeable Haemophilus influenzae
Outer membrane protein
Journal
Vaccine
ISSN: 1873-2518
Titre abrégé: Vaccine
Pays: Netherlands
ID NLM: 8406899
Informations de publication
Date de publication:
10 01 2020
10 01 2020
Historique:
received:
12
12
2018
revised:
23
09
2019
accepted:
04
10
2019
pubmed:
2
11
2019
medline:
10
2
2021
entrez:
1
11
2019
Statut:
ppublish
Résumé
Moraxella catarrhalis and nontypeable Haemophilus influenzae are important bacterial causes of otitis media in children and respiratory diseases in adults. Lipooligosaccharide (LOS) from M. catarrhalis and outer membrane protein 26 (OMP26) from NTHi are major surface antigens identified as potential vaccine components against these organisms. We previously constructed M. catarrhalis in which LOS is truncated, but contains a structure common to the three known serotypes of M. catarrhalis. OMP26 is known to enhance clearance of NTHi following vaccination in animal models, so was chosen as the carrier protein. In this study, we conjugated wild-type and truncated M. catarrhalis detoxified-LOS to a recombinant modified OMP26, rOMP26VTAL. Vaccination of mice with these conjugates resulted in a significant increase in anti-LOS and anti-rOMP26VTAL IgG levels. Importantly, mouse antisera showed complement-mediated bactericidal activity against all M. catarrhalis serotype A and B strains and a NTHi strain tested. Serotypes A & B make up more than 90% of isolates. These data suggest that the LOS and OMP based conjugate can be used as vaccine components and require further investigation in animal models.
Identifiants
pubmed: 31668366
pii: S0264-410X(19)31377-5
doi: 10.1016/j.vaccine.2019.10.014
pii:
doi:
Substances chimiques
Antigens, Bacterial
0
Bacterial Outer Membrane Proteins
0
Bacterial Vaccines
0
Haemophilus Vaccines
0
Immunoglobulin G
0
Lipopolysaccharides
0
Vaccines, Conjugate
0
lipid-linked oligosaccharides
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
309-317Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.