Transcriptomics and proteomics reveal two waves of translational repression during the maturation of malaria parasite sporozoites.
Animals
Anopheles
/ parasitology
Chromatography, Liquid
Epigenetic Repression
/ genetics
Gene Expression Profiling
Gene Expression Regulation, Developmental
/ genetics
Humans
Malaria
Malaria, Falciparum
Mosquito Vectors
/ parasitology
Oocysts
/ genetics
Plasmodium falciparum
/ genetics
Plasmodium yoelii
/ genetics
Proteome
/ metabolism
Proteomics
RNA, Messenger
/ metabolism
Rodentia
Salivary Glands
/ parasitology
Sporozoites
/ genetics
Tandem Mass Spectrometry
Transcriptome
/ genetics
Up-Regulation
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
31 10 2019
31 10 2019
Historique:
received:
29
05
2019
accepted:
09
10
2019
entrez:
2
11
2019
pubmed:
2
11
2019
medline:
4
3
2020
Statut:
epublish
Résumé
Plasmodium sporozoites are transmitted from infected mosquitoes to mammals, and must navigate the host skin and vasculature to infect the liver. This journey requires distinct proteomes. Here, we report the dynamic transcriptomes and proteomes of both oocyst sporozoites and salivary gland sporozoites in both rodent-infectious Plasmodium yoelii parasites and human-infectious Plasmodium falciparum parasites. The data robustly define mRNAs and proteins that are upregulated in oocyst sporozoites (UOS) or upregulated in infectious sporozoites (UIS) within the salivary glands, including many that are essential for sporozoite functions in the vector and host. Moreover, we find that malaria parasites use two overlapping, extensive, and independent programs of translational repression across sporozoite maturation to temporally regulate protein expression. Together with gene-specific validation experiments, these data indicate that two waves of translational repression are implemented and relieved at different times during sporozoite maturation, migration and infection, thus promoting their successful development and vector-to-host transition.
Identifiants
pubmed: 31673027
doi: 10.1038/s41467-019-12936-6
pii: 10.1038/s41467-019-12936-6
pmc: PMC6823429
doi:
Substances chimiques
Proteome
0
RNA, Messenger
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
4964Subventions
Organisme : NIAID NIH HHS
ID : R01 AI132359
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI123341
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM087221
Pays : United States
Organisme : NCRR NIH HHS
ID : S10 RR027584
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI134956
Pays : United States
Organisme : NIAID NIH HHS
ID : K25 AI119229
Pays : United States
Organisme : NIAID NIH HHS
ID : K22 AI101039
Pays : United States
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