Principles and Practices of Hybridization Capture Experiments to Study Long Noncoding RNAs That Act on Chromatin.
Journal
Cold Spring Harbor perspectives in biology
ISSN: 1943-0264
Titre abrégé: Cold Spring Harb Perspect Biol
Pays: United States
ID NLM: 101513680
Informations de publication
Date de publication:
01 11 2019
01 11 2019
Historique:
entrez:
3
11
2019
pubmed:
5
11
2019
medline:
2
10
2020
Statut:
epublish
Résumé
The diverse roles of cellular RNAs can be studied by purifying RNAs of interest together with the biomolecules they bind. Biotinylated antisense oligonucleotides that hybridize specifically to the RNA of interest provide a general approach to develop affinity reagents for these experiments. Such oligonucleotides can be used to enrich endogenous RNAs from cross-linked chromatin extracts to study the genomic binding sites of RNAs. These hybridization capture protocols are evolving modular experiments that are compatible with a range of cross-linkers and conditions. This review discusses the principles of these hybridization capture experiments as well as considerations and controls necessary to interpret the resulting data without being misled by artifactual signals.
Identifiants
pubmed: 31676573
pii: 11/11/a032276
doi: 10.1101/cshperspect.a032276
pmc: PMC6824240
pii:
doi:
Substances chimiques
Chromatin
0
RNA, Long Noncoding
0
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
Copyright © 2019 Cold Spring Harbor Laboratory Press; all rights reserved.
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