Proteomics Profiling of Autologous Blood and Semen Exosomes from HIV-infected and Uninfected Individuals Reveals Compositional and Functional Variabilities.
HIV
blood
exosomes
infectious disease
semen
viruses
Journal
Molecular & cellular proteomics : MCP
ISSN: 1535-9484
Titre abrégé: Mol Cell Proteomics
Pays: United States
ID NLM: 101125647
Informations de publication
Date de publication:
01 2020
01 2020
Historique:
received:
25
05
2019
revised:
18
10
2019
pubmed:
5
11
2019
medline:
21
10
2020
entrez:
3
11
2019
Statut:
ppublish
Résumé
Blood and semen are important body-fluids that carry exosomes for bioinformation transmission. Therefore, characterization of their proteomes is necessary for understanding body-fluid-specific physiologic and pathophysiologic functions. Using systematic multifactorial proteomic profiling, we characterized the proteomes of exosomes and exosome-free fractions from autologous blood and semen from three HIV-uninfected and three HIV-infected participants (total of 24 samples). We identified exosome-based protein signatures specific to blood and semen along with HIV-induced tissue-dependent proteomic perturbations. We validated our findings with samples from 16 additional donors and showed that unlike blood exosomes (BE), semen exosomes (SE) are enriched in clusterin. SE but not BE promote Protein·Nucleic acid binding and increase cell adhesion irrespective of HIV infection. This is the first comparative study of the proteome of autologous BE and SE. The proteins identified may be developed as biomarkers applicable to different fields of medicine, including reproduction and infectious diseases.
Identifiants
pubmed: 31676584
pii: S1535-9476(20)30007-4
doi: 10.1074/mcp.RA119.001594
pmc: PMC6944229
pii:
doi:
Substances chimiques
Biomarkers
0
Proteome
0
RNA, Viral
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
78-100Subventions
Organisme : NIAID NIH HHS
ID : R01 AI136740
Pays : United States
Organisme : NIDA NIH HHS
ID : R24 DA021471
Pays : United States
Organisme : NIDA NIH HHS
ID : R01 DA042348
Pays : United States
Organisme : NIDA NIH HHS
ID : R01 DA050169
Pays : United States
Organisme : NIAID NIH HHS
ID : P30 AI110527
Pays : United States
Organisme : NIAID NIH HHS
ID : T32 AI007343
Pays : United States
Organisme : NIDA NIH HHS
ID : R00 DA024558
Pays : United States
Organisme : NIDA NIH HHS
ID : R03 DA037779
Pays : United States
Organisme : NIDA NIH HHS
ID : R24 DA036420
Pays : United States
Organisme : BLRD VA
ID : I01 BX000207
Pays : United States
Organisme : NIAID NIH HHS
ID : R56 AI122960
Pays : United States
Organisme : NIDA NIH HHS
ID : R03 DA033892
Pays : United States
Informations de copyright
© 2020 Kaddour et al.
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