Adrenergic chromaffin cells are adrenergic even in the absence of epinephrine.
adrenal
chromaffin
epinephrine
exocytosis
norepinephrine
secretion
Journal
Journal of neurochemistry
ISSN: 1471-4159
Titre abrégé: J Neurochem
Pays: England
ID NLM: 2985190R
Informations de publication
Date de publication:
02 2020
02 2020
Historique:
received:
03
09
2019
revised:
24
10
2019
accepted:
29
10
2019
pubmed:
5
11
2019
medline:
1
7
2020
entrez:
3
11
2019
Statut:
ppublish
Résumé
Adrenal chromaffin cells release epinephrine (EPI) and norepinephrine (NE) into the bloodstream as part of the homeostatic response to situations like stress. Here we utilized EPI-deficient mice generated by knocking out (KO) the phenylethanolamine N-methyltransferase (Pnmt) gene. These Pnmt-KO mice were bred to homozygosis but displayed no major phenotype. The lack of EPI was partially compensated by an increase in NE, suggesting that EPI storage was optimized in adrenergic cells. Electron microscopy showed that despite the lack of EPI, chromaffin granules retain their shape and general appearance. This indicate that granules from adrenergic or noradrenergic cells preserve their characteristics even though they contain only NE. Acute insulin injection largely reduced the EPI content in wild-type animals, with a minimal reduction in NE, whereas there was only a partial reduction in NE content in Pnmt-KO mice. The analysis of exocytosis by amperometry revealed a reduction in the quantum size (-30%) and I
Substances chimiques
Phenylethanolamine N-Methyltransferase
EC 2.1.1.28
Norepinephrine
X4W3ENH1CV
Epinephrine
YKH834O4BH
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
299-314Informations de copyright
© 2019 International Society for Neurochemistry.
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