First report of Cryptosporidium hominis in a freshwater sponge.

Animals Cryptosporidium / isolation & purification Fresh Water / microbiology Oocysts Phylogeny Polymerase Chain Reaction Porifera / parasitology
Biofilms Cryptosporidium IFT PCR Protozoa Sponges

Journal

The Science of the total environment
ISSN: 1879-1026
Titre abrégé: Sci Total Environ
Pays: Netherlands
ID NLM: 0330500

Informations de publication

Date de publication:
15 Jan 2020
Historique:
received: 20 08 2019
accepted: 12 09 2019
pubmed: 5 11 2019
medline: 10 1 2020
entrez: 3 11 2019
Statut: ppublish

Résumé

Identification of Cryptosporidium oocyst is essential in ensuring water quality fit for human use, consumption, and recreation. This communication proposes the supplemental analysis of substrate-associated biofilms, in particular, freshwater sponges in improving case finding of waterborne-protozoan pathogens (WBPP) in environmental aquatic samples. In this study, a small portion of a mature freshwater sponge under the Genus Spongilla was subjected to microscopic and molecular analysis to identify the presence of Cryptosporidium. Microscopic screening with modified Kinyoun's staining (MK) and microscopic confirmation using direct antibody fluorescent testing (IFT) returned with Cryptosporidium spp. positive findings. Molecular investigation resulted in the confirmation of Cryptosporidium hominis upon sequencing of PCR products and phylogenetic analysis. This is the first report of a pathogenic protozoan, C. hominis isolated from a freshwater sponge. The results of this study provide evidence of the value of expanding water quality assessment strategies to the analysis of substrate-associated biofilms and sponges in improving case finding of WBPP in natural aquatic environments.

Identifiants

DOI: 10.1016/j.scitotenv.2019.134447 PMID: 31677419
pubmed: 31677419
pii: S0048-9697(19)34438-9
doi: 10.1016/j.scitotenv.2019.134447
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

134447

Informations de copyright

Copyright © 2019 Elsevier B.V. All rights reserved.

Auteurs

Frederick R Masangkay (FR)

Biomedical Sciences Program, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, Thailand; Department of Medical Technology, Institute of Arts and Sciences, Far Eastern University-Manila, Manila 1015, Philippines. Electronic address: frederick_masangkay2002@yahoo.com.

Giovanni D Milanez (GD)

Biomedical Sciences Program, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, Thailand; Department of Medical Technology, Institute of Arts and Sciences, Far Eastern University-Manila, Manila 1015, Philippines.

Amalia Tsiami (A)

London Geller College of Hospitality and Tourism, University of West London, St Mary's Road, Ealing, London W5 5RF, United Kingdom.

Voravuth Somsak (V)

Biomedical Sciences Program, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, Thailand.

Manas Kotepui (M)

Biomedical Sciences Program, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, Thailand.

Jitbanjong Tangpong (J)

Biomedical Sciences Program, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, Thailand. Electronic address: rjitbanj@wu.ac.th.

Panagiotis Karanis (P)

University of Cologne, Faculty of Medicine and University Hospital Cologne, Cologne, Germany; University of Nicosia Medical School, Anatomy Centre, P.O. Box 24005, CY-1700, Nicosia, 2408, Cyprus.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › First report of Cryptosporidium hominis in a...
questionsmedicales.fr › Eucaryotes › Alveolata › Apicomplexa › Coccidia › Eimeriida › Cryptosporidiidae › Cryptosporidium › First report of Cryptosporidium hominis in a...
questionsmedicales.fr › Environnement et santé publique › Environnement › Eau douce › First report of Cryptosporidium hominis in a...
questionsmedicales.fr › Phénomènes physiologiques › Croissance et développement › Morphogenèse › Métamorphose biologique › Étapes du cycle de vie › Oocystes › First report of Cryptosporidium hominis in a...
questionsmedicales.fr › Sciences de l'information › Phylogenèse › First report of Cryptosporidium hominis in a...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › First report of Cryptosporidium hominis in a...
questionsmedicales.fr › Eucaryotes › Animaux › Invertébrés › Porifera › First report of Cryptosporidium hominis in a...