Plant-Derived Exosomal Nanoparticles Inhibit Pathogenicity of Porphyromonas gingivalis.
Bacteriology
Biochemistry
Biological Sciences
Immunology
Microbiology
Molecular Biology
Oral Microbiology
Journal
iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038
Informations de publication
Date de publication:
22 Nov 2019
22 Nov 2019
Historique:
received:
13
02
2019
revised:
01
07
2019
accepted:
16
10
2019
pubmed:
5
11
2019
medline:
5
11
2019
entrez:
4
11
2019
Statut:
ppublish
Résumé
Plant exosomes protect plants against infection; however, whether edible plant exosomes can protect mammalian hosts against infection is not known. In this study, we show that ginger exosome-like nanoparticles (GELNs) are selectively taken up by the periodontal pathogen Porphyromonas gingivalis in a GELN phosphatidic acid (PA) dependent manner via interactions with hemin-binding protein 35 (HBP35) on the surface of P. gingivalis. Compared with PA (34:2), PA (34:1) did not interact with HBP35, indicating that the degree of unsaturation of PA plays a critical role in GELN-mediated interaction with HBP35. On binding to HBP35, pathogenic mechanisms of P. gingivalis were significantly reduced following interaction with GELN cargo molecules, including PA and miRs. These cargo molecules interacted with multiple pathogenic factors in the recipient bacteria simultaneously. Using edible plant exosome-like nanoparticles as a potential therapeutic agent to prevent/treat chronic periodontitis was further demonstrated in a mouse model.
Identifiants
pubmed: 31678913
pii: S2589-0042(19)30412-2
doi: 10.1016/j.isci.2019.10.032
pmc: PMC6838522
pii:
doi:
Types de publication
Journal Article
Langues
eng
Pagination
308-327Subventions
Organisme : NIGMS NIH HHS
ID : P20 GM113226
Pays : United States
Organisme : NIAAA NIH HHS
ID : P50 AA024337
Pays : United States
Organisme : NIDCR NIH HHS
ID : R01 DE011111
Pays : United States
Organisme : NIDCR NIH HHS
ID : R01 DE012505
Pays : United States
Commentaires et corrections
Type : ErratumIn
Informations de copyright
Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.
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