Single-cell proteomics reveals changes in expression during hair-cell development.
RNA sequencing
actin
cell biology
chicken
development
developmental biology
hair cells
mass spectrometry
single cells
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
04 11 2019
04 11 2019
Historique:
received:
02
08
2019
accepted:
01
11
2019
pubmed:
5
11
2019
medline:
9
4
2020
entrez:
5
11
2019
Statut:
epublish
Résumé
Hearing and balance rely on small sensory hair cells that reside in the inner ear. To explore dynamic changes in the abundant proteins present in differentiating hair cells, we used nanoliter-scale shotgun mass spectrometry of single cells, each ~1 picoliter, from utricles of embryonic day 15 chickens. We identified unique constellations of proteins or protein groups from presumptive hair cells and from progenitor cells. The single-cell proteomes enabled the de novo reconstruction of a developmental trajectory using protein expression levels, revealing proteins that greatly increased in expression during differentiation of hair cells (e.g., OCM, CRABP1, GPX2, AK1, GSTO1) and those that decreased during differentiation (e.g., TMSB4X, AGR3). Complementary single-cell transcriptome profiling showed corresponding changes in mRNA during maturation of hair cells. Single-cell proteomics data thus can be mined to reveal features of cellular development that may be missed with transcriptomics.
Identifiants
pubmed: 31682227
doi: 10.7554/eLife.50777
pii: 50777
pmc: PMC6855842
doi:
pii:
Substances chimiques
Proteome
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIH HHS
ID : R01 DC011034
Pays : United States
Organisme : NIDCD NIH HHS
ID : R01 DC011034
Pays : United States
Organisme : U.S. Department of Energy
ID : Laboratory Directed Research and Development Program (Earth & Biological Sciences Directorate Mission Seed)
Pays : International
Organisme : NINDS NIH HHS
ID : P30 NS061800
Pays : United States
Organisme : Laboratory Directed Research and Development Program at PNNL
ID : Earth & Biological Sciences Directorate Mission Seed
Pays : International
Organisme : NCI NIH HHS
ID : R33 CA225248
Pays : United States
Organisme : NIH HHS
ID : R01 DC015201
Pays : United States
Organisme : NIH HHS
ID : R33 CA225248
Pays : United States
Organisme : Hearing Health Foundation
ID : Hearing Restoration Project
Pays : International
Organisme : NIDCD NIH HHS
ID : R01 DC015201
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA069533
Pays : United States
Déclaration de conflit d'intérêts
YZ, MS, JK, DC, RK, SH, PB No competing interests declared, DE is affiliated with Amgen Inc. The author has no other competing interests to declare
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