Ketone body receptor GPR43 regulates lipid metabolism under ketogenic conditions.


Journal

Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876

Informations de publication

Date de publication:
19 11 2019
Historique:
pubmed: 7 11 2019
medline: 3 4 2020
entrez: 6 11 2019
Statut: ppublish

Résumé

Ketone bodies, including β-hydroxybutyrate and acetoacetate, are important alternative energy sources during energy shortage. β-Hydroxybutyrate also acts as a signaling molecule via specific G protein-coupled receptors (GPCRs); however, the specific associated GPCRs and physiological functions of acetoacetate remain unknown. Here we identified acetoacetate as an endogenous agonist for short-chain fatty acid (SCFA) receptor GPR43 by ligand screening in a heterologous expression system. Under ketogenic conditions, such as starvation and low-carbohydrate diets, plasma acetoacetate levels increased markedly, whereas plasma and cecal SCFA levels decreased dramatically, along with an altered gut microbiota composition. In addition,

Identifiants

pubmed: 31685604
pii: 1912573116
doi: 10.1073/pnas.1912573116
pmc: PMC6876247
doi:

Substances chimiques

Ffar2 protein, mouse 0
Ketone Bodies 0
Ligands 0
Receptors, G-Protein-Coupled 0
Lipoprotein Lipase EC 3.1.1.34

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

23813-23821

Informations de copyright

Copyright © 2019 the Author(s). Published by PNAS.

Déclaration de conflit d'intérêts

The authors declare no competing interest.

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Auteurs

Junki Miyamoto (J)

Department of Applied Biological Science, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu-shi, 183-8509 Tokyo, Japan.
Japan Agency for Medical Research and Development-Core Research for Evolutionary Medical Science and Technology (AMED-CREST), Japan Agency for Medical Research and Development, Chiyoda-ku, 100-0004 Tokyo, Japan.

Ryuji Ohue-Kitano (R)

Department of Applied Biological Science, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu-shi, 183-8509 Tokyo, Japan.
Japan Agency for Medical Research and Development-Core Research for Evolutionary Medical Science and Technology (AMED-CREST), Japan Agency for Medical Research and Development, Chiyoda-ku, 100-0004 Tokyo, Japan.
Department of Endocrinology, Metabolism, and Hypertension, Clinical Research Institute, National Hospital Organization Kyoto Medical Center, 612-8555 Kyoto, Japan.

Hiromi Mukouyama (H)

Department of Applied Biological Science, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu-shi, 183-8509 Tokyo, Japan.

Akari Nishida (A)

Department of Applied Biological Science, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu-shi, 183-8509 Tokyo, Japan.

Keita Watanabe (K)

Department of Applied Biological Science, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu-shi, 183-8509 Tokyo, Japan.

Miki Igarashi (M)

Department of Applied Biological Science, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu-shi, 183-8509 Tokyo, Japan.

Junichiro Irie (J)

Japan Agency for Medical Research and Development-Core Research for Evolutionary Medical Science and Technology (AMED-CREST), Japan Agency for Medical Research and Development, Chiyoda-ku, 100-0004 Tokyo, Japan.
Department of Endocrinology, Metabolism, and Nephrology, School of Medicine, Keio University, Shinjuku-ku, 160-8582 Tokyo, Japan.

Gozoh Tsujimoto (G)

Department of Genomic Drug Discovery Science, Graduate School of Pharmaceutical Sciences, Kyoto University, 606-8501 Kyoto, Japan.

Noriko Satoh-Asahara (N)

Department of Endocrinology, Metabolism, and Hypertension, Clinical Research Institute, National Hospital Organization Kyoto Medical Center, 612-8555 Kyoto, Japan.

Hiroshi Itoh (H)

Japan Agency for Medical Research and Development-Core Research for Evolutionary Medical Science and Technology (AMED-CREST), Japan Agency for Medical Research and Development, Chiyoda-ku, 100-0004 Tokyo, Japan.
Department of Endocrinology, Metabolism, and Nephrology, School of Medicine, Keio University, Shinjuku-ku, 160-8582 Tokyo, Japan.

Ikuo Kimura (I)

Department of Applied Biological Science, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu-shi, 183-8509 Tokyo, Japan; ikimura@cc.tuat.ac.jp.
Japan Agency for Medical Research and Development-Core Research for Evolutionary Medical Science and Technology (AMED-CREST), Japan Agency for Medical Research and Development, Chiyoda-ku, 100-0004 Tokyo, Japan.

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Classifications MeSH