Evaluation of serological assays available in a biosafety level 2 laboratory and their application for survey of Middle East respiratory syndrome coronavirus among livestock in Ethiopia.
Animals
Camelus
/ virology
Cattle
Chlorocebus aethiops
Containment of Biohazards
Coronavirus Infections
/ epidemiology
Ethiopia
/ epidemiology
Goats
HEK293 Cells
Humans
Middle East Respiratory Syndrome Coronavirus
/ immunology
Neutralization Tests
/ methods
Seroepidemiologic Studies
Sheep
Spike Glycoprotein, Coronavirus
Vero Cells
Vesiculovirus
Ethiopia
Middle East respiratory syndrome
camel
coronavirus
serological survey
Journal
The Journal of veterinary medical science
ISSN: 1347-7439
Titre abrégé: J Vet Med Sci
Pays: Japan
ID NLM: 9105360
Informations de publication
Date de publication:
26 Dec 2019
26 Dec 2019
Historique:
pubmed:
7
11
2019
medline:
6
5
2020
entrez:
6
11
2019
Statut:
ppublish
Résumé
A serological survey of Middle East respiratory syndrome coronavirus (MERS-CoV) was conducted among dromedary camels and herbivorous animals sharing the same pasturage in Ethiopia. The pseudotyped vesicular stomatitis virus coated with the spike protein of MERS-CoV was used in virus neutralization (VN) tests performed in a biosafety level (BSL)-2 laboratory. The results were similar to those obtained from the VN test using live MERS-CoV and were more sensitive than the ELISA performed using synthetic MERS S1 fragment as the antigen as well as the competitive ELISA performed using a monoclonal antibody against MERS-CoV. According to the comprehensive results of the four types of serodiagnosis methods, positive antibodies were detected only in dromedary camels and the remaining herbivorous animals were not infected with the virus. Moreover, using the present procedure, serological tests for MERS-CoV can be conducted even in BSL 2 laboratory.
Identifiants
pubmed: 31685722
doi: 10.1292/jvms.19-0436
pmc: PMC6943330
doi:
Substances chimiques
Spike Glycoprotein, Coronavirus
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1887-1891Références
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