Strontium enhances proliferation and osteogenic behavior of bone marrow stromal cells of mesenchymal and ectomesenchymal origins in vitro.


Journal

Clinical and experimental dental research
ISSN: 2057-4347
Titre abrégé: Clin Exp Dent Res
Pays: United States
ID NLM: 101692332

Informations de publication

Date de publication:
10 2019
Historique:
received: 13 05 2019
revised: 02 06 2019
accepted: 04 06 2019
entrez: 6 11 2019
pubmed: 7 11 2019
medline: 7 11 2019
Statut: epublish

Résumé

To investigate the effect of increasing Strontium (Sr) concentrations on the growth and osteogenic behavior of human bone marrow stromal cells (BMSCs) from mesenchymal (i.e., fibula) and ectomesenchymal (i.e., mandible) embryonic origins. Fibula and mandible BMSCs were cultured in media without (Ctrl) or with Sr in four diverse concentrations: Sr1, 11.3 × 10 Fibula and mandible BMSCs cultured with Sr4 showed increased proliferation rate, and osteocalcin and osteopontin gene expression together with more evident formation of mineralized nodules, compared all other Sr concentrations. For both cell populations, Sr4 led to lower ALP activity, and ALP gene expression, compared with the other Sr concentrations. BMSCs from mesenchymal (i.e., fibula) and ectomesenchymal (i.e., mandible) embryonic origins showed increased cellular proliferation and osteogenic behavior when cultured with Sr4, in vitro.

Identifiants

pubmed: 31687189
doi: 10.1002/cre2.221
pii: CRE2221
pmc: PMC6820574
doi:

Substances chimiques

Osteocalcin 104982-03-8
Osteopontin 106441-73-0
Alkaline Phosphatase EC 3.1.3.1
Strontium YZS2RPE8LE

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Pagination

541-550

Informations de copyright

©2019 The Authors. Clinical and Experimental Dental Research published by John Wiley & Sons Ltd.

Déclaration de conflit d'intérêts

There are no conflicts of interest.

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Auteurs

Carolina Bizelli-Silveira (C)

Department of Dentistry and Oral Health, Faculty of Health Aarhus University Aarhus Denmark.
Department of Orthopaedic Surgery Aarhus University Hospital Aarhus Denmark.

Lisbeth Ann Abildtrup (LA)

Department of Dentistry and Oral Health, Faculty of Health Aarhus University Aarhus Denmark.

Rubens Spin-Neto (R)

Department of Dentistry and Oral Health, Faculty of Health Aarhus University Aarhus Denmark.

Morten Foss (M)

Interdisciplinary Nanoscience Center (iNANO), Department of Physics and Astronomy, Faculty of Science and Technology Aarhus University Aarhus Denmark.

Kjeld Søballe (K)

Department of Orthopaedic Surgery Aarhus University Hospital Aarhus Denmark.

David Christian Evar Kraft (DCE)

Department of Dentistry and Oral Health, Faculty of Health Aarhus University Aarhus Denmark.

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Classifications MeSH