Development and evaluation of high-resolution melting curve analysis for rapid detection and subtyping of Blastocystis and comparison the results with sequencing.
18S ribosomal RNA gene
Blastocystis
High-resolution melting curve analysis
Subtypes
Journal
Parasitology research
ISSN: 1432-1955
Titre abrégé: Parasitol Res
Pays: Germany
ID NLM: 8703571
Informations de publication
Date de publication:
Dec 2019
Dec 2019
Historique:
received:
15
06
2019
accepted:
25
09
2019
pubmed:
7
11
2019
medline:
28
1
2020
entrez:
7
11
2019
Statut:
ppublish
Résumé
Blastocystis is a prevalent parasite that has a wide distribution. In order to design HRM real-time PCR, primers were selected from SSU rRNA gene to amplify specific fragment with different melting temperatures for each subtype of Blastocystis. Subsequently, HRM real-time PCR was performed and melting curve analysis was done by Rotor-Gene Q software. The results of HRM real-time PCR was then compared with sequence results of "barcoding region" of SSU rRNA gene of Blastocystis. To evaluate sensitivity of test, 10-fold serial dilutions of the parasite were prepared from ~ 10
Identifiants
pubmed: 31691003
doi: 10.1007/s00436-019-06486-5
pii: 10.1007/s00436-019-06486-5
doi:
Substances chimiques
DNA Primers
0
DNA, Protozoan
0
Types de publication
Evaluation Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
3469-3478Références
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