Crosslinked Poly(N-Isopropylacrylamide)-Based Microfibers as Cell Manipulation Materials with Prompt Cell Detachment.

Acrylic Resins / chemistry Animals Biocompatible Materials / chemistry Cell Adhesion Cross-Linking Reagents / chemistry Mice Microscopy, Confocal NIH 3T3 Cells Particle Size Surface Properties Temperature
fast cell detachment microfibers thermoresponsive polymers volume expansion

Journal

Macromolecular rapid communications
ISSN: 1521-3927
Titre abrégé: Macromol Rapid Commun
Pays: Germany
ID NLM: 9888239

Informations de publication

Date de publication:
Dec 2019
Historique:
received: 04 09 2019
revised: 06 10 2019
pubmed: 7 11 2019
medline: 16 4 2020
entrez: 7 11 2019
Statut: ppublish

Résumé

Stimuli-responsive smart materials are a key to the realization of next-generation medical technologies. Among them, the temperature-responsive polymer poly(N-isopropylacrylamide) (PNIPAAm) is attracting particular attention because it is easy to use in physiological conditions. PNIPAAm-grafted surfaces can undergo temperature-modulated cell adhesion and detachment without proteolytic enzymes, and can be used as cell-separating materials through selective cell adhesion/detachment. However, cell detachment at reduced temperatures is problematic because it takes several hours. A novel thermoresponsive crosslinked microfiber system that can greatly reduce the cell detachment time is introduced in this study. The crosslinked fibers provide temperature-dependent volume change, and enable cell detachment within 10 min of reducing the temperature, which is one-sixth of the time required in previous studies. The prompt cell detachment is thought to arise from a completely new mechanism derived from fiber swelling. This system will make a significant contribution as a novel cell manipulating system for next-generation medical technology.

Identifiants

DOI: 10.1002/marc.201900464 PMID: 31692103
pubmed: 31692103
doi: 10.1002/marc.201900464
doi:

Substances chimiques

Acrylic Resins 0
Biocompatible Materials 0
Cross-Linking Reagents 0
poly-N-isopropylacrylamide 25189-55-3

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e1900464

Subventions

Organisme : JST SENTAN
ID : JPMJSN16B3

Informations de copyright

© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Références

A. S. Hoffman, P. S. Stayton, Prog. Polym. Sci. 2007, 32, 922.
D. Roy, J. N. Cambre, B. S. Sumerlin, Prog. Polym. Sci. 2010, 35, 278.
K. Nagase, M. Yamato, H. Kanazawa, T. Okano, Biomaterials 2018, 153, 27.
X. Lin, D. Tang, Z. Yu, Q. Feng, J. Mater. Chem. B 2014, 2, 651.
G. Pan, Q. Guo, Y. Ma, H. Yang, B. Li, Angew. Chem., Int. Ed. 2013, 52, 6907.
Q. Zhang, P. Dong, L. Chen, X. Wang, S. Lu, J. Biomed. Mater. Res., Part A 2014, 102, 76.
J. ter Schiphorst, J. Saez, D. Diamond, F. Benito-Lopez, A. P. Schenning, Lab Chip 2018, 18, 699.
M. Krogsgaard, M. A. Behrens, J. S. Pedersen, H. Birkedal, Biomacromolecules 2013, 14, 297.
M. Chen, X. He, K. Wang, D. He, S. Yang, P. Qiu, S. Chen, J. Mater. Chem. B 2014, 2, 428.
M. L. Tebaldi, C. M. Oda, L. O. Monteiro, A. L. de Barros, C. J. Santos, D. C. F. Soares, J. Magn. Magn. Mater. 2018, 461, 116.
M. Heskins, J. E. Guillet, J. Macromol. Sci. Chem. 1968, 2, 1441.
Y. Maeda, T. Higuchi, I. Ikeda, Langmuir 2000, 16, 7503.
K. Nagase, J. Kobayashi, A. Kikuchi, Y. Akiyama, H. Kanazawa, T. Okano, Biomacromolecules 2014, 15, 3846.
K. Nagase, Y. Hatakeyama, T. Shimizu, K. Matsuura, M. Yamato, N. Takeda, T. Okano, Biomacromolecules 2013, 14, 3423.
K. Nagase, Y. Sakurada, S. Onizuka, T. Iwata, M. Yamato, N. Takeda, T. Okano, Acta Biomater. 2017, 53, 81.
Y. Akiyama, A. Kikuchi, M. Yamato, T. Okano, Langmuir 2004, 20, 5506.
Y. Arisaka, J. Kobayashi, M. Yamato, Y. Akiyama, T. Okano, Biomaterials 2013, 34, 4214.
K. Nagase, Y. Hatakeyama, T. Shimizu, K. Matsuura, M. Yamato, N. Takeda, T. Okano, Biomacromolecules 2015, 16, 532.
A. Mizutani, A. Kikuchi, M. Yamato, H. Kanazawa, T. Okano, Biomaterials 2008, 29, 2073.
K. Nagase, R. Shukuwa, T. Onuma, M. Yamato, N. Takeda, T. Okano, J. Mater. Chem. B 2017, 5, 5924.
J. Li, T. Kaku, Y. Tokura, K. Matsukawa, K. Homma, T. Nishimoto, Y. Hiruta, A. Mizutani Akimoto, K. Nagase, H. Kanazawa, S. Shiratori, Biomacromolecules 2019, 20, 1748.
E. Ayano, Y. Okada, C. Sakamoto, H. Kanazawa, A. Kikuchi, T. Okano, J. Chromatogr. A 2006, 1119, 51.
A. Kushida, M. Yamato, C. Konno, A. Kikuchi, Y. Sakurai, T. Okano, J. Biomed. Mater. Res. 1999, 45, 355.
J. R. Kuhn, T. D. Pollard, Biophys. J. 2005, 88, 1387.

Auteurs

Tomomi Konishi (T)

Center for Material Design Science, School of Integrated Design Engineering, Keio University, 3-14-1 Hiyoshi, Yokohama, 223-8522, Japan.

Aya Mizutani Akimoto (A)

Department of Materials Engineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Tokyo, 113-8656, Japan.

Taihei Nishimoto (T)

Faculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Minato, Tokyo, 105-8512, Japan.

Yuki Tokura (Y)

Center for Material Design Science, School of Integrated Design Engineering, Keio University, 3-14-1 Hiyoshi, Yokohama, 223-8522, Japan.
ORCID: 0000-0001-7303-4189

Mizuki Tenjimbayashi (M)

Center for Material Design Science, School of Integrated Design Engineering, Keio University, 3-14-1 Hiyoshi, Yokohama, 223-8522, Japan.

Kenta Homma (K)

Department of Materials Engineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Tokyo, 113-8656, Japan.

Ko Matsukawa (K)

Department of Materials Engineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Tokyo, 113-8656, Japan.

Taisei Kaku (T)

Center for Material Design Science, School of Integrated Design Engineering, Keio University, 3-14-1 Hiyoshi, Yokohama, 223-8522, Japan.

Yuki Hiruta (Y)

Center for Material Design Science, School of Integrated Design Engineering, Keio University, 3-14-1 Hiyoshi, Yokohama, 223-8522, Japan.

Kenichi Nagase (K)

Faculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Minato, Tokyo, 105-8512, Japan.
ORCID: 0000-0002-6575-0107

Hideko Kanazawa (H)

Faculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Minato, Tokyo, 105-8512, Japan.
ORCID: 0000-0003-2550-470X

Seimei Shiratori (S)

Center for Material Design Science, School of Integrated Design Engineering, Keio University, 3-14-1 Hiyoshi, Yokohama, 223-8522, Japan.
ORCID: 0000-0001-9807-3555

Articles similaires

Evaluating the efficacy of telesurgery with dual console SSI Mantra Surgical Robotic System: experiment on animal model and clinical trials.

Sudhir Prem Srivastava, Vishwajyoti Pascual Srivastava, Avinesh Singh et al.
1.00
Robotic Surgical Procedures Animals Humans Telemedicine Models, Animal

Odour generalisation and detection dog training.

Lyn Caldicott, Thomas W Pike, Helen E Zulch et al.
1.00
Animals Odorants Dogs Generalization, Psychological Smell

FBXO22 inhibits colitis and colorectal carcinogenesis by regulating the degradation of the S2448-phosphorylated form of mTOR.

Minle Li, Xuan Chen, Pengfei Qu et al.
1.00
Animals TOR Serine-Threonine Kinases Colorectal Neoplasms Colitis Mice

Use of organic material provided by an automatic enrichment device by weaner pigs and its influence on tail lesions.

Philipp Heseker, Jeanette Probst, Stefanie Ammer et al.
1.00
Animals Tail Swine Behavior, Animal Animal Husbandry

Classifications MeSH

questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomédicaux et dentaires › Polymères › Matières plastiques › Résines synthétiques › Résines acryliques › Crosslinked Poly(N-Isopropylacrylamide)-Based...
questionsmedicales.fr › Eucaryotes › Animaux › Crosslinked Poly(N-Isopropylacrylamide)-Based...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomédicaux et dentaires › Matériaux biocompatibles › Crosslinked Poly(N-Isopropylacrylamide)-Based...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Adhérence cellulaire › Crosslinked Poly(N-Isopropylacrylamide)-Based...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Produits chimiques de laboratoire › Indicateurs et réactifs › Réactifs réticulants › Crosslinked Poly(N-Isopropylacrylamide)-Based...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Crosslinked Poly(N-Isopropylacrylamide)-Based...
questionsmedicales.fr › Techniques d'investigation › Microscopie confocale › Crosslinked Poly(N-Isopropylacrylamide)-Based...
questionsmedicales.fr › Cellules › Cellules du tissu conjonctif › Fibroblastes › Cellules 3T3 › Cellules NIH 3T3 › Crosslinked Poly(N-Isopropylacrylamide)-Based...
questionsmedicales.fr › Phénomènes chimiques › Taille de particule › Crosslinked Poly(N-Isopropylacrylamide)-Based...
questionsmedicales.fr › Phénomènes chimiques › Propriétés de surface › Crosslinked Poly(N-Isopropylacrylamide)-Based...
questionsmedicales.fr › Environnement et santé publique › Environnement › Concepts météorologiques › Atmosphère › Temps (météorologie) › Température › Crosslinked Poly(N-Isopropylacrylamide)-Based...