Deciphering the transcriptomic insight during organogenesis in Castor (
Auxins
Callus
Castor
DEGs
Organogenesis
RNA-seq
Journal
3 Biotech
ISSN: 2190-572X
Titre abrégé: 3 Biotech
Pays: Germany
ID NLM: 101565857
Informations de publication
Date de publication:
Nov 2019
Nov 2019
Historique:
received:
26
07
2019
accepted:
18
10
2019
entrez:
8
11
2019
pubmed:
7
11
2019
medline:
7
11
2019
Statut:
ppublish
Résumé
Cultivation of the castor crop is hindered by various factors and one of the approaches for genetic improvement of the crop is through exploitation of biotechnological tools. Response of castor tissues to in vitro culture is poor which necessitated this study on understanding the molecular basis of organogenesis in cultured tissues of castor, through de novo transcriptome analysis and by comparing with jatropha and sunflower having good regeneration ability. Transcriptome profiling analysis was carried out with hypocotyl explants from castor, jatropha and cotyledons from sunflower cultured on MS media supplemented with different concentrations of hormones. Differentially expressed genes during dedifferentiation and organogenic differentiation stages of callus included components of auxin and cytokinin signaling, secondary metabolite synthesis, genes encoding transcription factors, receptor kinases and protein kinases. In castor, many genes involved in auxin biosynthesis and homeostasis like WAT1, vacuolar transporter genes, transcription factors like short root like protein were down-regulated while genes like DELLA were up-regulated accounting for regeneration recalcitrance. Validation of 62 DEGs through qRT-PCR showed a consensus of 77.4% of the genes expressed. Overall study provides set of genes involved in the process of organogenesis in three oilseed crops which forms a basis for understanding and improving the efficiency of plant regeneration and genetic transformation in castor.
Identifiants
pubmed: 31696039
doi: 10.1007/s13205-019-1960-9
pii: 1960
pmc: PMC6825063
doi:
Types de publication
Journal Article
Langues
eng
Pagination
434Informations de copyright
© King Abdulaziz City for Science and Technology 2019.
Déclaration de conflit d'intérêts
Conflict of interestThe authors declare that they have no conflicts of interest.
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