Analytical performance of canine acylated and unacylated ghrelin enzyme-linked immunosorbent assays.
accuracy
dog
precision
storage
validation
Journal
Veterinary clinical pathology
ISSN: 1939-165X
Titre abrégé: Vet Clin Pathol
Pays: United States
ID NLM: 9880575
Informations de publication
Date de publication:
Dec 2019
Dec 2019
Historique:
received:
14
02
2018
revised:
14
03
2019
accepted:
29
03
2019
pubmed:
7
11
2019
medline:
3
6
2020
entrez:
8
11
2019
Statut:
ppublish
Résumé
Ghrelin is a major appetite-stimulating hormone. It circulates as acylated ghrelin (AG) and unacylated ghrelin (UAG), which could have different metabolic actions in obesity. Our objective was to study the analytical performance of two new canine AG and UAG ELISAs using blood samples from healthy, normal-weight dogs. Additionally, the effect of a protease inhibitor (PI) on short-term sample storage was analyzed. The intra- and inter-assay precision for low, intermediate, and high AG and UAG concentrations, accuracy, limits of quantification (LQ), and detection limit (DL) of a blank sample were determined in ten healthy dogs. To study the effects of a PI on ghrelin concentrations, and AG and UAG concentrations were compared in five canine plasma samples stored for 1 month with (PI+), without (PI-), and with PI added at sample thawing (PI+th). The intra- and inter-assay coefficients of variation were 1.8%-5.7% and 2.9%-6.4% for the AG assay, and 0.8%-7.5% and 2.8%-13.4% for the UAG assay, respectively. Accuracy analyses showed nonsignificant deviation from linearity for the AG (R The AG and UAG ELISA assays had acceptable precision, accuracy, lower LQ, and DLs, but upper LQ could not be established. An influence of the PI on short-term storage was not detectable. Long-term storage ± PI was not evaluated and should be investigated further.
Substances chimiques
Ghrelin
0
Serine Proteinase Inhibitors
0
Sulfones
0
4-(2-aminoethyl)benzenesulfonylfluoride
34284-75-8
Types de publication
Evaluation Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
748-753Subventions
Organisme : Den Danske Kennelklub
Organisme : Fondet for Sygdomsbekaempelse hos Vore Familiedyr
Organisme : Bertin Pharma
Informations de copyright
© 2019 American Society for Veterinary Clinical Pathology.
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