Thermodynamic Analysis of the Uptake of a Protein in a Spherical Polyelectrolyte Brush.
ITC
Spherical polyelectrolyte brushes
enthalpy-entropy compensation (EEC)
proteins
thermodynamics
Journal
Macromolecular rapid communications
ISSN: 1521-3927
Titre abrégé: Macromol Rapid Commun
Pays: Germany
ID NLM: 9888239
Informations de publication
Date de publication:
Jan 2020
Jan 2020
Historique:
received:
15
08
2019
revised:
19
09
2019
pubmed:
8
11
2019
medline:
30
5
2020
entrez:
8
11
2019
Statut:
ppublish
Résumé
A thermodynamic study of the adsorption of Human Serum Albumin (HSA) onto spherical polyelectrolyte brushes (SPBs) by isothermal titration calorimetry (ITC) is presented. The SPBs are composed of a solid polystyrene core bearing long chains of poly(acrylic acid). ITC measurements done at different temperatures and ionic strengths lead to a full set of thermodynamicbinding constants together with the enthalpies and entropies of binding. The adsorption of HSA onto SPBs is described with a two-step model. The free energy of binding ΔGb depends only weakly on temperature because of a marked compensation of enthalpy by entropy. Studies of the adsorbed HSA by Fourier transform infrared spectroscopy (FT-IR) demonstrate no significant disturbance in the secondary structure of the protein. The quantitative analysis demonstrates that counterion release is the major driving force for adsorption in a process where proteins become multivalent counterions of the polyelectrolyte chains upon adsorption. A comparison with the analysis of other sets of data related to the binding of HSA to polyelectrolytes demonstrates that the cancellation of enthalpy and entropy is a general phenomenon that always accompanies the binding of proteins to polyelectrolytes dominated by counterion release.
Identifiants
pubmed: 31697416
doi: 10.1002/marc.201900421
doi:
Substances chimiques
Acrylic Resins
0
Polyelectrolytes
0
Polystyrenes
0
Serum Albumin
0
carbopol 940
4Q93RCW27E
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1900421Subventions
Organisme : Deutsche Forschungsgemeinschaft
Organisme : National Science Foundation Research Triangle Materials Research Science and Engineering Center
ID : NSF MRSEC
Organisme : National Science Foundation Research Triangle Materials Research Science and Engineering Center
ID : DMR-11211107
Informations de copyright
© 2019 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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