The Role of CXCL13 and CXCL9 in Early Breast Cancer.


Journal

Clinical breast cancer
ISSN: 1938-0666
Titre abrégé: Clin Breast Cancer
Pays: United States
ID NLM: 100898731

Informations de publication

Date de publication:
02 2020
Historique:
received: 25 04 2019
revised: 30 07 2019
accepted: 26 08 2019
pubmed: 9 11 2019
medline: 30 3 2021
entrez: 9 11 2019
Statut: ppublish

Résumé

Chemokines, cytokines in the immune microenvironment of tumors, may be associated with patient outcome. We assessed the impact of CXCL13 and CXCL9 on disease-free (DFS) and overall survival (OS), in an attempt to retrospectively evaluate both T and B cell function in the microenvironment of primary tumors from patients with breast cancer. Formalin-fixed paraffin-embedded tissue blocks from patients with intermediate/high-risk, early breast cancer, treated with sequential adjuvant epirubicin, paclitaxel, and cyclophosphamide methotrexate fluorouracil within a randomized trial, were tested for CXCL13 and CXCL9 messenger RNA expression; 557 patients with adequate tissue were eligible for the analysis. CXCL13 was correlated with CXCL9 (rho = 0.52; P < .001). High-expressing CXL13 and CXCL9 tumors had higher Ki67 and tumor infiltrating lymphocyte density (P-values < .001). High CXCL9 expression was an unfavorable prognosticator for OS among all patients (hazard ratio [HR], 1.73; P = .021), whereas it showed favorable significance for both DFS and OS in patients with triple negative disease (HR, 0.29; P = .027 and HR, 0.32; P = .045). High CXCL13 conferred longer DFS and OS among patients with luminal-human epidermal growth factor receptor 2 disease (HR, 0.31; P = .013 and HR, 0.25; P = .005). Patients with low CXCL13 and high CXCL9 expression had shorter DFS and OS compared with those with high expression of both chemokines (HR, 1.63; P = .006 and HR, 1.61; P = .016). Both biomarkers were associated with poor prognosis characteristics and with tumor infiltrating lymphocyte density. High CXCL9 conferred an improved prognosis in the triple negative subtype, whereas high CXCL13 was associated with improved outcome in the luminal-human epidermal growth factor receptor 2 subtype. Chemokines can be associated with breast cancer subtype and outcome. These data should be evaluated prospectively.

Sections du résumé

BACKGROUND
Chemokines, cytokines in the immune microenvironment of tumors, may be associated with patient outcome. We assessed the impact of CXCL13 and CXCL9 on disease-free (DFS) and overall survival (OS), in an attempt to retrospectively evaluate both T and B cell function in the microenvironment of primary tumors from patients with breast cancer.
MATERIALS AND METHODS
Formalin-fixed paraffin-embedded tissue blocks from patients with intermediate/high-risk, early breast cancer, treated with sequential adjuvant epirubicin, paclitaxel, and cyclophosphamide methotrexate fluorouracil within a randomized trial, were tested for CXCL13 and CXCL9 messenger RNA expression; 557 patients with adequate tissue were eligible for the analysis.
RESULTS
CXCL13 was correlated with CXCL9 (rho = 0.52; P < .001). High-expressing CXL13 and CXCL9 tumors had higher Ki67 and tumor infiltrating lymphocyte density (P-values < .001). High CXCL9 expression was an unfavorable prognosticator for OS among all patients (hazard ratio [HR], 1.73; P = .021), whereas it showed favorable significance for both DFS and OS in patients with triple negative disease (HR, 0.29; P = .027 and HR, 0.32; P = .045). High CXCL13 conferred longer DFS and OS among patients with luminal-human epidermal growth factor receptor 2 disease (HR, 0.31; P = .013 and HR, 0.25; P = .005). Patients with low CXCL13 and high CXCL9 expression had shorter DFS and OS compared with those with high expression of both chemokines (HR, 1.63; P = .006 and HR, 1.61; P = .016).
CONCLUSIONS
Both biomarkers were associated with poor prognosis characteristics and with tumor infiltrating lymphocyte density. High CXCL9 conferred an improved prognosis in the triple negative subtype, whereas high CXCL13 was associated with improved outcome in the luminal-human epidermal growth factor receptor 2 subtype. Chemokines can be associated with breast cancer subtype and outcome. These data should be evaluated prospectively.

Identifiants

pubmed: 31699671
pii: S1526-8209(19)30667-6
doi: 10.1016/j.clbc.2019.08.008
pii:
doi:

Substances chimiques

Biomarkers, Tumor 0
CXCL13 protein, human 0
CXCL9 protein, human 0
Chemokine CXCL13 0
Chemokine CXCL9 0
Receptors, Estrogen 0
Receptors, Progesterone 0
ERBB2 protein, human EC 2.7.10.1
Receptor, ErbB-2 EC 2.7.10.1

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e36-e53

Informations de copyright

Copyright © 2019 Elsevier Inc. All rights reserved.

Auteurs

Evangelia Razis (E)

Third Department of Medical Oncology, Hygeia Hospital, Athens, Greece. Electronic address: e.razis@hygeia.gr.

Konstantine T Kalogeras (KT)

Translational Research Section, Hellenic Cooperative Oncology Group, Athens, Greece; Laboratory of Molecular Oncology, Hellenic Foundation for Cancer Research/Aristotle University of Thessaloniki, Thessaloniki, Greece.

Ioannis Kotsantis (I)

Section of Medical Oncology, Department of Internal Medicine, Attikon University Hospital, National and Kapodistrian University of Athens School of Medicine, Athens, Greece.

Georgia-Angeliki Koliou (GA)

Section of Biostatistics, Hellenic Cooperative Oncology Group, Data Office, Athens, Greece.

Kyriaki Manousou (K)

Section of Biostatistics, Hellenic Cooperative Oncology Group, Data Office, Athens, Greece.

Ralph Wirtz (R)

STRATIFYER Molecular Pathology GmbH, Cologne, Germany.

Elke Veltrup (E)

STRATIFYER Molecular Pathology GmbH, Cologne, Germany.

Helen Patsea (H)

Department of Pathology, IASSO General Hospital, Athens, Greece.

Nikiforita Poulakaki (N)

Breast Surgery Clinic, Euroclinic, Athens, Greece.

Dimitrios Dionysopoulos (D)

Department of Medical Oncology, Papageorgiou Hospital, Aristotle University of Thessaloniki, School of Health Sciences, Faculty of Medicine, Thessaloniki, Greece.

Stavroula Pervana (S)

Department of Pathology, Papageorgiou Hospital, Thessaloniki, Greece.

Helen Gogas (H)

First Department of Medicine, Laiko General Hospital, National and Kapodistrian University of Athens School of Medicine, Athens, Greece.

Angelos Koutras (A)

Division of Oncology, Department of Medicine, University Hospital, University of Patras Medical School, Patras, Greece.

George Pentheroudakis (G)

Department of Medical Oncology, Ioannina University Hospital, Ioannina, Greece.

Christos Christodoulou (C)

Second Department of Medical Oncology, Metropolitan Hospital, Piraeus, Greece.

Helena Linardou (H)

Oncology Unit, Metropolitan Hospital, Piraeus, Greece.

Kitty Pavlakis (K)

Pathology Department, National and Kapodistrian University of Athens School of Medicine, Athens, Greece.

Triantafyllia Koletsa (T)

Department of Pathology, Aristotle University of Thessaloniki, School of Health Sciences, Faculty of Medicine, Thessaloniki, Greece.

Dimitrios Pectasides (D)

Oncology Section, Second Department of Internal Medicine, Hippokration Hospital, Athens, Greece.

Flora Zagouri (F)

Department of Clinical Therapeutics, Alexandra Hospital, National and Kapodistrian University of Athens School of Medicine, Athens, Greece.

George Fountzilas (G)

Laboratory of Molecular Oncology, Hellenic Foundation for Cancer Research/Aristotle University of Thessaloniki, Thessaloniki, Greece; Aristotle University of Thessaloniki, Thessaloniki, Greece.

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Classifications MeSH