Methyl Ether-Derivatized Sterols and Coprostanol Produced via Thermochemolysis Using Tetramethylammonium Hydroxide (TMAH).
coprostanol
sterols
tetramethylammonium hydroxide (TMAH)
thermochemolysis
Journal
Molecules (Basel, Switzerland)
ISSN: 1420-3049
Titre abrégé: Molecules
Pays: Switzerland
ID NLM: 100964009
Informations de publication
Date de publication:
07 Nov 2019
07 Nov 2019
Historique:
received:
07
10
2019
revised:
31
10
2019
accepted:
05
11
2019
entrez:
10
11
2019
pubmed:
11
11
2019
medline:
2
4
2020
Statut:
epublish
Résumé
Sterols are widely distributed in nature from lipids in organisms to sediments. As a conventional method, extraction and derivatization with TMS have been applied for sterol analysis, requiring a long preparation time for gas chromatography-mass spectrometry analysis. In this study, for sterol analysis, thermochemolysis using tetramethylammonium hydroxide (TMAH) was applied. This method performs hydrolysis and methylation simultaneously; thus, free and ether-bonding sterols can be analyzed as sterol methyl ethers in a relatively short time period. A sediment sample from a tideland (the Yatsu tideland, Japan) was analyzed using the TMAH method, and we detected more than 10 sterols, which include cholest-5-en-3
Identifiants
pubmed: 31703423
pii: molecules24224040
doi: 10.3390/molecules24224040
pmc: PMC6928973
pii:
doi:
Substances chimiques
Cholestenes
0
Quaternary Ammonium Compounds
0
Cholestanol
8M308U816E
dinosterol
91FGT6HJ59
tetramethylammonium
H0W55235FC
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Japan Science Society
ID : The Sasakawa Scientific Research Grant
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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