Plasma Levels and Tissue Expression of Selected Cytokines, Metalloproteinases and Tissue Inhibitors in Patients With Cervical Cancer.
Adenocarcinoma
/ blood
Adult
Carcinoma, Squamous Cell
/ blood
Cytokines
/ analysis
Female
Humans
Macrophage Colony-Stimulating Factor
/ analysis
Matrix Metalloproteinase 2
/ analysis
Matrix Metalloproteinase 9
/ analysis
Metalloproteases
/ analysis
Middle Aged
Tissue Inhibitor of Metalloproteinase-1
/ analysis
Tissue Inhibitor of Metalloproteinase-2
/ analysis
Tissue Inhibitor of Metalloproteinases
/ analysis
Uterine Cervical Dysplasia
/ blood
Uterine Cervical Neoplasms
/ blood
Vascular Endothelial Growth Factor A
/ analysis
Young Adult
Tumor marker
cervical cancer
macrophage colony-stimulating factor
matrix metalloproteinases
tissue inhibitors of matrix metalloproteinases
vascular endothelial growth factor
Journal
Anticancer research
ISSN: 1791-7530
Titre abrégé: Anticancer Res
Pays: Greece
ID NLM: 8102988
Informations de publication
Date de publication:
Nov 2019
Nov 2019
Historique:
received:
15
09
2019
revised:
10
10
2019
accepted:
14
10
2019
entrez:
10
11
2019
pubmed:
11
11
2019
medline:
15
11
2019
Statut:
ppublish
Résumé
Cytokines, metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) take part in many processes involved in tumor progression and invasion such as degradation of the extracellular matrix, influence on immune cells associated with tumor tissue, and angiogenesis. Thus, the aim of this study was to compare the concentration of plasma levels and tissue expression of macrophage colony-stimulating factor (M-CSF), vascular endothelial growth factor (VEGF), matrix metalloproteinases (MMP)-2 and MMP9, and their tissue inhibitors TIMP1 and TIMP2 in patients with cervical cancer, patients with high-grade cervical intraepithelial dysplasia (CIN3) and patients with ectropion. Concentration and expression of all tested parameters was measured in serum with enzyme-linked immunosorbent assay (ELISA) and in tissue with immunohistochemistry method. The epithelial expression of M-CSF and TIMP1 in cancer tissue was much stronger as compared to that in ectropion and CIN3. In the case of MMP2, lack of or weak expression in epithelial cells was observed in all tested groups. Our studies showed statistical differences of tested parameters in tissue expression and in plasma concentrations in patients with cervical cancer, patients with CIN3 and patients with ectropion. Moreover, data revealed positive correlation between plasma level and cervical cancer cell expression of VEGF. Our findings indicate a potential role of all the proteins tested here in cervical cancer diagnosis, especially VEGF. However, further studies will show whether they play a role in the progression of cancerous changes in epithelial tissue of the cervix.
Sections du résumé
BACKGROUND
BACKGROUND
Cytokines, metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) take part in many processes involved in tumor progression and invasion such as degradation of the extracellular matrix, influence on immune cells associated with tumor tissue, and angiogenesis. Thus, the aim of this study was to compare the concentration of plasma levels and tissue expression of macrophage colony-stimulating factor (M-CSF), vascular endothelial growth factor (VEGF), matrix metalloproteinases (MMP)-2 and MMP9, and their tissue inhibitors TIMP1 and TIMP2 in patients with cervical cancer, patients with high-grade cervical intraepithelial dysplasia (CIN3) and patients with ectropion.
PATIENTS AND METHODS
METHODS
Concentration and expression of all tested parameters was measured in serum with enzyme-linked immunosorbent assay (ELISA) and in tissue with immunohistochemistry method.
RESULTS
RESULTS
The epithelial expression of M-CSF and TIMP1 in cancer tissue was much stronger as compared to that in ectropion and CIN3. In the case of MMP2, lack of or weak expression in epithelial cells was observed in all tested groups. Our studies showed statistical differences of tested parameters in tissue expression and in plasma concentrations in patients with cervical cancer, patients with CIN3 and patients with ectropion. Moreover, data revealed positive correlation between plasma level and cervical cancer cell expression of VEGF.
CONCLUSION
CONCLUSIONS
Our findings indicate a potential role of all the proteins tested here in cervical cancer diagnosis, especially VEGF. However, further studies will show whether they play a role in the progression of cancerous changes in epithelial tissue of the cervix.
Identifiants
pubmed: 31704874
pii: 39/11/6403
doi: 10.21873/anticanres.13854
doi:
Substances chimiques
CSF1 protein, human
0
Cytokines
0
TIMP1 protein, human
0
TIMP2 protein, human
0
Tissue Inhibitor of Metalloproteinase-1
0
Tissue Inhibitor of Metalloproteinases
0
VEGFA protein, human
0
Vascular Endothelial Growth Factor A
0
Tissue Inhibitor of Metalloproteinase-2
127497-59-0
Macrophage Colony-Stimulating Factor
81627-83-0
Metalloproteases
EC 3.4.-
Matrix Metalloproteinase 2
EC 3.4.24.24
Matrix Metalloproteinase 9
EC 3.4.24.35
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
6403-6412Informations de copyright
Copyright© 2019, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.