Inactivation of foot-and-mouth disease virus in epithelium samples for safe transport and processing in low-containment laboratories.
Foot-and-mouth disease virus
Inactivation
Sample transport
Tissue samples
Journal
Journal of virological methods
ISSN: 1879-0984
Titre abrégé: J Virol Methods
Pays: Netherlands
ID NLM: 8005839
Informations de publication
Date de publication:
02 2020
02 2020
Historique:
received:
11
12
2018
revised:
04
11
2019
accepted:
05
11
2019
pubmed:
11
11
2019
medline:
16
3
2021
entrez:
10
11
2019
Statut:
ppublish
Résumé
During a foot-and-mouth disease (FMD) outbreak, transport and testing of potentially infectious samples, including epithelium from suspect lesions, presents a biosafety risk, particularly in FMD-free countries. Therefore, treatment to inactivate virus prior to transport is important. Tongue epithelium from cattle infected with FMD virus (FMDV) serotype O (O ALG/3/2014 - Lineage O/ME-SA/Ind-2001d) or A (A IRN/22/2015 - Lineage A/ASIA/G-VII) was incubated in RNAlater, RNA Shield or phosphate-buffered saline (pH 7.4) at room temperature for 2, 6, 24 or 48 h. After incubation, tissues were homogenised and tested by virus titration. Viral RNA in the homogenate was quantified by RT-qPCR, used for sequencing, and transfected into LFBK
Identifiants
pubmed: 31705919
pii: S0166-0934(18)30603-7
doi: 10.1016/j.jviromet.2019.113770
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
113770Informations de copyright
Copyright © 2019. Published by Elsevier B.V.