The effect of surgery on patellar tendinopathy: Novel use of MRI questions the exploitability of the rat collagenase model to humans.
Animal model
Collagenase
Patellar tendinopathy
Rat
Surgery
Journal
The Knee
ISSN: 1873-5800
Titre abrégé: Knee
Pays: Netherlands
ID NLM: 9430798
Informations de publication
Date de publication:
Dec 2019
Dec 2019
Historique:
received:
20
05
2019
revised:
20
10
2019
accepted:
22
10
2019
pubmed:
11
11
2019
medline:
1
4
2020
entrez:
11
11
2019
Statut:
ppublish
Résumé
patellar tendinopathy is an overuse condition most commonly affecting jumping athletes. Surgery is reserved for refractory cases; however, it lacks high level clinical evidence and basic science to support its use. The purpose of this study was to determine the biomechanical and histological response of surgical excision on patellar tendinopathy in the rat collagenase tendinopathy model and correlate MRI findings. Forty-eight Long Evans rats were divided into three groups: i) no patellar tendinopathy with surgical excision, ii) patella tendinopathy with surgical excision, and iii) patellar tendinopathy with no surgical excision. Endpoints included histology, mechanical testing, and MRI pre- and post-surgical intervention at one and four weeks. No difference in failure load or histological grading was seen between the groups at all time points. MRIs showed initial loss of tendon continuity followed by complete healing with elongated and thickened tendons in all groups. While other research has reported immunohistochemistry and histology of collagenase-induced tendinopathy may be correlated with human pathogenesis, the novel MRI findings from our study suggest that the rat collagenase tendinopathy surgical model may be limited when extrapolating to humans. Further work is needed to determine if any correlation exists between the dosing, location, and animal effect of the collagenase injection model with MRI findings. This is needed before any collagenase model can be used to determine the effect of surgery in the pathogenic response to patella tendinopathy.
Sections du résumé
BACKGROUND
BACKGROUND
patellar tendinopathy is an overuse condition most commonly affecting jumping athletes. Surgery is reserved for refractory cases; however, it lacks high level clinical evidence and basic science to support its use. The purpose of this study was to determine the biomechanical and histological response of surgical excision on patellar tendinopathy in the rat collagenase tendinopathy model and correlate MRI findings.
METHODS
METHODS
Forty-eight Long Evans rats were divided into three groups: i) no patellar tendinopathy with surgical excision, ii) patella tendinopathy with surgical excision, and iii) patellar tendinopathy with no surgical excision. Endpoints included histology, mechanical testing, and MRI pre- and post-surgical intervention at one and four weeks.
RESULTS
RESULTS
No difference in failure load or histological grading was seen between the groups at all time points. MRIs showed initial loss of tendon continuity followed by complete healing with elongated and thickened tendons in all groups.
CONCLUSIONS
CONCLUSIONS
While other research has reported immunohistochemistry and histology of collagenase-induced tendinopathy may be correlated with human pathogenesis, the novel MRI findings from our study suggest that the rat collagenase tendinopathy surgical model may be limited when extrapolating to humans. Further work is needed to determine if any correlation exists between the dosing, location, and animal effect of the collagenase injection model with MRI findings. This is needed before any collagenase model can be used to determine the effect of surgery in the pathogenic response to patella tendinopathy.
Identifiants
pubmed: 31706694
pii: S0968-0160(19)30243-1
doi: 10.1016/j.knee.2019.10.017
pii:
doi:
Substances chimiques
Collagenases
EC 3.4.24.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1182-1191Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.