Lentiviral Vector Production in Suspension Culture Using Serum-Free Medium for the Transduction of CAR-T Cells.
Lentiviral vectors
Packaging cells
Producer cells
Serum-free medium
Suspension cells
Transient transfection
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
11
11
2019
pubmed:
11
11
2019
medline:
1
1
2021
Statut:
ppublish
Résumé
The production of lentiviral vectors (LVs) in human embryonic kidney 293 (HEK293) cells using serum-free medium in a suspension culture for the transduction of chimeric antigen receptor T-cells (CAR-T) can be achieved by different methods. This chapter describes LV production by transient transfection, induction of stable packaging cell lines, and induction of stable producer cell lines.
Identifiants
pubmed: 31707669
doi: 10.1007/978-1-0716-0146-4_6
doi:
Substances chimiques
Culture Media, Serum-Free
0
Receptors, Chimeric Antigen
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM