Multiparametric MR Investigation of Proteoglycan Diffusivity, T
T2-weighted MRS
chemical exchange saturation transfer
diffusion-weighted MRS
extracellular matrix
intervertebral disc degeneration
proteoglycan
Journal
Journal of magnetic resonance imaging : JMRI
ISSN: 1522-2586
Titre abrégé: J Magn Reson Imaging
Pays: United States
ID NLM: 9105850
Informations de publication
Date de publication:
05 2020
05 2020
Historique:
received:
19
07
2019
revised:
11
10
2019
accepted:
11
10
2019
pubmed:
12
11
2019
medline:
15
5
2021
entrez:
12
11
2019
Statut:
ppublish
Résumé
Proteoglycan (PG) is a major component of the intervertebral disc extracellular matrix (ECM) that acts to hydrate the disc nucleus. Early detection of PG degradation is valuable for both diagnosis and preclinical research of intervertebral disc degeneration (IVDD). To compare different MR techniques for detecting early degradative changes of PG in IVDD. Prospective. Glycosaminoglycan (GAG) phantom/bovine discs with papain injection and human cadaveric discs. 7T/diffusion-weighted MR spectroscopy (DW-MRS), T DW-MRS, T T-tests were conducted to measure the differences of PG properties between pre- and post-enzyme injection. Linear regression and mixed-effects models were used to assess the associations among different PG properties as well as the degeneration grades in human cadaveric discs. In bovine discs, PG diffusivity increased most rapidly after the enzyme was injected into the disc nucleus (12 hours postinjection, t = 5.76, P = 0.0007). The PG T PG diffusivity is a direct biomarker for early ECM degradation, while PG distribution can be an indirect biomarker for early IVDD. 2 Technical Efficacy Stage: 2 J. Magn. Reson. Imaging 2020;51:1390-1400.
Sections du résumé
BACKGROUND
Proteoglycan (PG) is a major component of the intervertebral disc extracellular matrix (ECM) that acts to hydrate the disc nucleus. Early detection of PG degradation is valuable for both diagnosis and preclinical research of intervertebral disc degeneration (IVDD).
PURPOSE
To compare different MR techniques for detecting early degradative changes of PG in IVDD.
STUDY TYPE
Prospective.
PHANTOM/SPECIMEN
Glycosaminoglycan (GAG) phantom/bovine discs with papain injection and human cadaveric discs.
FIELD STRENGTH/SEQUENCES
7T/diffusion-weighted MR spectroscopy (DW-MRS), T
ASSESSMENT
DW-MRS, T
STATISTICAL TESTS
T-tests were conducted to measure the differences of PG properties between pre- and post-enzyme injection. Linear regression and mixed-effects models were used to assess the associations among different PG properties as well as the degeneration grades in human cadaveric discs.
RESULTS
In bovine discs, PG diffusivity increased most rapidly after the enzyme was injected into the disc nucleus (12 hours postinjection, t = 5.76, P = 0.0007). The PG T
DATA CONCLUSION
PG diffusivity is a direct biomarker for early ECM degradation, while PG distribution can be an indirect biomarker for early IVDD.
LEVEL OF EVIDENCE
2 Technical Efficacy Stage: 2 J. Magn. Reson. Imaging 2020;51:1390-1400.
Substances chimiques
Proteoglycans
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1390-1400Informations de copyright
© 2019 International Society for Magnetic Resonance in Medicine.
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