Circulating Endometrial Cells: A New Source of Information on Endometriosis Dynamics.

circulating endometrial cells endometriosis liquid biopsy menstrual cycle rare cells

Journal

Journal of clinical medicine
ISSN: 2077-0383
Titre abrégé: J Clin Med
Pays: Switzerland
ID NLM: 101606588

Informations de publication

Date de publication:
11 Nov 2019
Historique:
received: 28 09 2019
revised: 22 10 2019
accepted: 04 11 2019
entrez: 14 11 2019
pubmed: 14 11 2019
medline: 14 11 2019
Statut: epublish

Résumé

The focus of the presented work was to isolate and characterize circulating endometrial cells (CECs) enriched from peripheral blood (PB) of patients with diagnosed endometriosis. The molecular characteristics of CECs could be supportive for an understanding of endometriosis pathogenesis and treatment decisions in the future. Blood samples ( CECs were present in 78.4% of the tested blood samples. In line with the revised American Fertility Society (rAFS) classification, CECs presence was confirmed in all the acknowledged endometriosis stages: minimal, mild, moderate, and severe. Surprisingly, CECs negativity rate was also reported for severe disease in 21.1% of cases. The CECs captured during MC phases displayed different cytomorphology, including epithelial, stromal, and stem cell-like characteristics. The highest CECs numbers were detected in the mid-secretory phase of MC, which corresponds to uterine lining decidualization. CECs captured during mid-secretory periods expressed genes The panel of the identified CEC genes could be tested in a prospective manner to confirm the role of CECs in endometriosis pathogenesis and diagnostics.

Identifiants

DOI: 10.3390/jcm8111938 PMID: 31717910 PMC: PMC6912292
pubmed: 31717910
pii: jcm8111938
doi: 10.3390/jcm8111938
pmc: PMC6912292
pii:
doi:

Types de publication

Journal Article

Langues

eng

Subventions

Organisme : DIP
ID : Grant No. RPDS.01.02.01-02-0074/15-02

Références

Am J Obstet Gynecol. 1952 Oct;64(4):780-806
pubmed: 12985717
Cochrane Database Syst Rev. 2016 Jul 13;7:CD012281
pubmed: 27405583
Int J Mol Sci. 2019 Jul 31;20(15):null
pubmed: 31370190
Eur J Obstet Gynecol Reprod Biol. 2014 Oct;181:267-74
pubmed: 25195200
Cochrane Database Syst Rev. 2015 Dec 23;(12):CD012019
pubmed: 26695425
Stem Cells. 2018 Jun;36(6):881-890
pubmed: 29450941
Am J Pathol. 1927 Mar;3(2):93-110.43
pubmed: 19969738
Front Surg. 2015 Jan 06;1:51
pubmed: 25593975
Fertil Steril. 2013 Mar 15;99(4):1135-45
pubmed: 23414923
Biomed Res Int. 2015;2015:130854
pubmed: 26240814
Cochrane Database Syst Rev. 2016 Apr 20;4:CD012165
pubmed: 27094925
J Am Med Assoc. 1937 Jun 12;108(24):2022-8
pubmed: 12255649
Am J Pathol. 2011 Apr;178(4):1782-91
pubmed: 21435458
Obstet Gynecol Clin North Am. 2003 Mar;30(1):1-19, vii
pubmed: 12699255
Front Surg. 2017 Jul 14;4:37
pubmed: 28770208
Obstet Gynecol Int. 2013;2013:527041
pubmed: 23843796
Cochrane Database Syst Rev. 2016 May 01;(5):CD012179
pubmed: 27132058
Hum Reprod Update. 2013 Sep-Oct;19(5):570-82
pubmed: 23727940
Reprod Med Biol. 2018 Feb 01;17(3):220-227
pubmed: 30013421
Chin Med J (Engl). 2017 Oct 5;130(19):2339-2345
pubmed: 28937041
Ann N Y Acad Sci. 2008 Apr;1127:106-15
pubmed: 18443337
Hum Reprod Update. 2010 Nov-Dec;16(6):651-74
pubmed: 20462942
Annu Rev Pathol. 2019 Sep 3;:
pubmed: 31479615

Auteurs

Eliska Pospisilova (E)

Department of Laboratory Genetics, Laboratory Diagnostics, Faculty Hospital Královské Vinohrady, Srobarova 50, 100 34 Prague 10, Czech Republic.
Department of Gynecology, Military University Hospital and 3rd Faculty of Medicine, U Vojenske nemocnice 1200, 169 02 Prague 6, Czech Republic.

Imrich Kiss (I)

Department of Laboratory Genetics, Laboratory Diagnostics, Faculty Hospital Královské Vinohrady, Srobarova 50, 100 34 Prague 10, Czech Republic.
Department of Gynecology, Military University Hospital and 3rd Faculty of Medicine, U Vojenske nemocnice 1200, 169 02 Prague 6, Czech Republic.
Department of Obstetrics and Gynecology, University Hospital, Faculty of Medicine Charles University, Alej Svobody 80, 301 66 Pilsen, Czech Republic.

Helena Souckova (H)

Department of Gynecology, Military University Hospital and 3rd Faculty of Medicine, U Vojenske nemocnice 1200, 169 02 Prague 6, Czech Republic.

Pavel Tomes (P)

Department of Obstetrics and Gynecology, University Hospital, Faculty of Medicine Charles University, Alej Svobody 80, 301 66 Pilsen, Czech Republic.

Jan Spicka (J)

Department of Laboratory Genetics, Laboratory Diagnostics, Faculty Hospital Královské Vinohrady, Srobarova 50, 100 34 Prague 10, Czech Republic.

Rafal Matkowski (R)

Cellpeutics Sp. z o.o., Duńska 9,54-424 Wrocław, Poland.
Department of Oncology, Wroclaw Medical University, Wybrzeże Ludwika Pasteura 1, 50-367 Wrocław, Poland and Wroclaw Comprehensive Cancer Center, Plac Ludwika Hirszfelda 12, 53-413 Wrocław, Poland.

Marcin Jedryka (M)

Cellpeutics Sp. z o.o., Duńska 9,54-424 Wrocław, Poland.
Department of Oncology, Wroclaw Medical University, Wybrzeże Ludwika Pasteura 1, 50-367 Wrocław, Poland and Wroclaw Comprehensive Cancer Center, Plac Ludwika Hirszfelda 12, 53-413 Wrocław, Poland.

Simone Ferrero (S)

Academic Unit of Obstetrics and Gynecology Ospedale Policlinico San Martino Genoa, Italy Department of Neurosciences, Rehabilitation, Ophthalmology, Genetics, Maternal and Child Health (DiNOGMI) University of Genoa Genoa, Italy Academic Unit of Gynecology and Obstetrics University of Genoa, 16 132 Genoa, Italy.
ORCID: 0000-0003-2225-5568

Vladimir Bobek (V)

Department of Laboratory Genetics, Laboratory Diagnostics, Faculty Hospital Královské Vinohrady, Srobarova 50, 100 34 Prague 10, Czech Republic.
Department of Oncology, Wroclaw Medical University, Wybrzeże Ludwika Pasteura 1, 50-367 Wrocław, Poland and Wroclaw Comprehensive Cancer Center, Plac Ludwika Hirszfelda 12, 53-413 Wrocław, Poland.
Department of Histology and Embryology, Wroclaw Medical University, L. Pasteur 1, 503 67 Wroclaw, Poland.
Department of Thoracic Surgery, Krajská zdravotní a.s. Hospital, 41100 Ústí nad Labem, Czech Republic.
3rd Department of Surgery University Hospital FN Motol and 1st Faculty of Medicine Charles University, V Uvalu 84, 150 06 Prague 5, Czech Republic.

Katarina Kolostova (K)

Department of Laboratory Genetics, Laboratory Diagnostics, Faculty Hospital Královské Vinohrady, Srobarova 50, 100 34 Prague 10, Czech Republic.
Department of Oncology, Wroclaw Medical University, Wybrzeże Ludwika Pasteura 1, 50-367 Wrocław, Poland and Wroclaw Comprehensive Cancer Center, Plac Ludwika Hirszfelda 12, 53-413 Wrocław, Poland.

Classifications MeSH