Neutrophil myeloperoxidase harbors distinct site-specific peculiarities in its glycosylation.

MPO anti-neutrophil cytoplasmic autoantibody (ANCA) autoimmune disease glycoproteomics glycosylation mannose-6-phosphate (M6P) mass spectrometry (MS) myeloperoxidase neutrophil paucimannose phosphomannose self-antigen

Journal

The Journal of biological chemistry
ISSN: 1083-351X
Titre abrégé: J Biol Chem
Pays: United States
ID NLM: 2985121R

Informations de publication

Date de publication:
27 12 2019
Historique:
received: 18 09 2019
revised: 08 11 2019
pubmed: 14 11 2019
medline: 23 6 2020
entrez: 14 11 2019
Statut: ppublish

Résumé

Anti-neutrophil cytoplasmic autoantibodies (ANCAs) are directed against lysosomal components of neutrophils. ANCAs directed to proteinase 3 and myeloperoxidase (MPO) in particular are associated with distinct forms of small vessel vasculitides. MPO is an abundant neutrophil-derived heme protein that is part of the antimicrobial defense system. The protein is typically present in the azurophilic granules of neutrophils, but a large portion may also enter the extracellular space. It remains unclear why MPO is frequently the target of antibody-mediated autoimmune responses. MPO is a homodimeric glycoprotein, posttranslationally modified with complex sugars at specific sites. Glycosylation can strongly influence protein function, affecting its folding, receptor interaction, and backbone accessibility. MPO potentially can be heavily modified as it harbors 5 putative

Identifiants

pubmed: 31719144
pii: S0021-9258(20)30039-9
doi: 10.1074/jbc.RA119.011098
pmc: PMC6937560
doi:

Substances chimiques

Glycopeptides 0
Polysaccharides 0
Peroxidase EC 1.11.1.7
Mannose PHA4727WTP

Banques de données

PDB
['1CXP']

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

20233-20245

Informations de copyright

© 2019 Reiding et al.

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Auteurs

Karli R Reiding (KR)

Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, University of Utrecht, 3584 CH Utrecht, The Netherlands k.r.reiding@uu.nl.
Netherlands Proteomics Center, 3584 CH Utrecht, The Netherlands.

Vojtech Franc (V)

Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, University of Utrecht, 3584 CH Utrecht, The Netherlands.
Netherlands Proteomics Center, 3584 CH Utrecht, The Netherlands.

Minke G Huitema (MG)

Department of Rheumatology and Clinical Immunology, University Medical Center Groningen, University of Groningen, 9700 AB Groningen, The Netherlands.

Elisabeth Brouwer (E)

Department of Rheumatology and Clinical Immunology, University Medical Center Groningen, University of Groningen, 9700 AB Groningen, The Netherlands.

Peter Heeringa (P)

Department of Pathology and Medical Biology, University Medical Center Groningen, University of Groningen, 9700 AB Groningen, The Netherlands.

Albert J R Heck (AJR)

Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, University of Utrecht, 3584 CH Utrecht, The Netherlands.
Netherlands Proteomics Center, 3584 CH Utrecht, The Netherlands.

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