Blocking connexin43 hemichannels protects mice against tumour necrosis factor-induced inflammatory shock.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
12 11 2019
Historique:
received: 25 07 2019
accepted: 22 10 2019
entrez: 14 11 2019
pubmed: 14 11 2019
medline: 11 11 2020
Statut: epublish

Résumé

Upon intravenous injection of tumour necrosis factor (TNF) in mice, a systemic inflammatory response syndrome (SIRS) is initiated, characterized by an acute cytokine storm and induction of vascular hyperpermeability. Connexin43 hemichannels have been implicated in various pathological conditions, e.g. ischemia and inflammation, and can lead to detrimental cellular outcomes. Here, we explored whether targeting connexin43 hemichannels could alleviate TNF-induced endothelial barrier dysfunction and lethality in SIRS. Therefore, we verified whether administration of connexin43-targeting-peptides affected survival, body temperature and vascular permeability in vivo. In vitro, TNF-effects on connexin43 hemichannel function were investigated by single-channel studies and Ca

Identifiants

pubmed: 31719598
doi: 10.1038/s41598-019-52900-4
pii: 10.1038/s41598-019-52900-4
pmc: PMC6851386
doi:

Substances chimiques

Chemokines 0
Connexin 43 0
Cytokines 0
Tumor Necrosis Factor-alpha 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

16623

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Auteurs

Tinneke Delvaeye (T)

VIB Center for Inflammation Research, Ghent, Belgium.
Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.
Department of Basic and Applied Medical Sciences, Ghent University, Ghent, Belgium.

Maarten A J De Smet (MAJ)

Department of Basic and Applied Medical Sciences, Ghent University, Ghent, Belgium.

Stijn Verwaerde (S)

Department of Basic and Applied Medical Sciences, Ghent University, Ghent, Belgium.

Elke Decrock (E)

Department of Basic and Applied Medical Sciences, Ghent University, Ghent, Belgium.

Aleksandra Czekaj (A)

VIB Center for Inflammation Research, Ghent, Belgium.
Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.

Roosmarijn E Vandenbroucke (RE)

VIB Center for Inflammation Research, Ghent, Belgium.
Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.

Kelly Lemeire (K)

VIB Center for Inflammation Research, Ghent, Belgium.
Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.

Amanda Gonçalves (A)

VIB Center for Inflammation Research, Ghent, Belgium.
Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.
VIB BioImaging Core, Ghent, Belgium.

Wim Declercq (W)

VIB Center for Inflammation Research, Ghent, Belgium.
Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.

Peter Vandenabeele (P)

VIB Center for Inflammation Research, Ghent, Belgium. peter.vandenabeele@irc.vib-ugent.be.
Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium. peter.vandenabeele@irc.vib-ugent.be.
Methusalem Program, Ghent University, Ghent, Belgium. peter.vandenabeele@irc.vib-ugent.be.

Dmitri V Krysko (DV)

Department of Human Structure and Repair, Ghent University, Ghent, Belgium. dmitri.krysko@ugent.be.

Luc Leybaert (L)

Department of Basic and Applied Medical Sciences, Ghent University, Ghent, Belgium. luc.leybaert@ugent.be.

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Classifications MeSH