Evaluation of The Expression Levels of Three Long Non-Coding RNAs in Multiple Sclerosis.
Autoimmune Disease
Gene Expression Profiling
Long Non-coding RNA
Multiple Sclerosis
Neurodegenerative Disease
Journal
Cell journal
ISSN: 2228-5806
Titre abrégé: Cell J
Pays: Iran
ID NLM: 101566618
Informations de publication
Date de publication:
Jul 2020
Jul 2020
Historique:
received:
23
11
2018
accepted:
12
03
2019
entrez:
14
11
2019
pubmed:
14
11
2019
medline:
14
11
2019
Statut:
ppublish
Résumé
Multiple sclerosis (MS) is a chronic disorder involving both inflammatory and neurodegenerative responses. Long non-coding RNAs (lncRNAs) have been had an emerging role as the biomarkers of different disorders, including autoimmune diseases. Previous studies have shown that NR_003531.3 (MEG3a), AC000061.1_201, and AC007182.6 play a role in the pathogenesis of human autoimmune diseases. However, the potential significance of these lncRNAs, as the diagnostic biomarkers of MS, has not been studied yet. We aimed to quantitatively evaluate the expression levels of NR_003531.3, AC000061.1_201, and AC007182.6 in peripheral blood samples of MS patients in comparison with healthy controls. In this case-control study, the blood samples from 20 MS patients and 10 healthy controls were collected. Total RNA was extracted, and the expression levels of three selected lncRNAs were quantitatively measured using the quantitative real time-polymerase chain reaction (qRT-PCR) method. We detected a significant down-regulation in the expression of NR_003531.3 in MS patients, while no marked changes were observed in the expression of AC000061.1_201 and AC007182.6 in patients compared with controls. Based on the receiver operating characteristic (ROC) curve analysis, NR_003531.3 could discriminate MS patients from healthy subjects effectively. Regarding the prognosis of MS patients, NR_003531.3 is significantly and inversely correlated with the expanded disability status scale (EDSS). The potential role of NR_003531.3 lncRNA as a diagnostic biomarker to distinguish MS patients is proposed. Prognostically, NR_003531.3 correlates with lower disability rates in MS patients.
Identifiants
pubmed: 31721530
doi: 10.22074/cellj.2020.6555
pmc: PMC6874792
doi:
Types de publication
Journal Article
Langues
eng
Pagination
165-170Informations de copyright
Copyright© by Royan Institute. All rights reserved.
Déclaration de conflit d'intérêts
There is no conflict of interest in this study.
Références
Mult Scler Relat Disord. 2017 May;14:35-45
pubmed: 28619429
Immunity. 2006 Feb;24(2):179-89
pubmed: 16473830
J Neuroimmunol. 2018 Nov 15;324:129-135
pubmed: 30170791
CNS Neurosci Ther. 2016 Apr;22(4):298-305
pubmed: 26842313
Cardiovasc Res. 2008 Sep 1;79(4):562-70
pubmed: 18511432
Biomed Pharmacother. 2016 Oct;83:905-911
pubmed: 27522004
J Neurochem. 2011 Feb;116(3):459-66
pubmed: 21128942
Indian J Clin Biochem. 2019 Oct;34(4):451-457
pubmed: 31686732
Arthritis Rheum. 2009 May;60(5):1472-83
pubmed: 19404966
Ann Neurol. 2011 Feb;69(2):292-302
pubmed: 21387374
Proc Natl Acad Sci U S A. 2009 Dec 22;106(51):21789-94
pubmed: 19955422
Can Med Assoc J. 1973 Jun 2;108(11):1356 passim
pubmed: 4704903
J Genet. 2017 Mar;96(1):109-118
pubmed: 28360395
Nat Commun. 2015 Apr 23;6:6932
pubmed: 25903499
Immunology. 2018 Apr;153(4):479-487
pubmed: 29030863
Cell Death Dis. 2017 Dec 13;8(12):3211
pubmed: 29238035
J Mol Neurosci. 2017 Dec;63(3-4):333-341
pubmed: 28967047
PLoS One. 2017 Nov 15;12(11):e0186795
pubmed: 29140972
J Genet. 2016 Dec;95(4):991-995
pubmed: 27994199
Gene. 2014 Jul 10;544(2):128-33
pubmed: 24792898
Am J Hum Genet. 2003 Mar;72(3):710-6
pubmed: 12557126
J Immunol. 2009 Sep 15;183(6):3672-81
pubmed: 19710457
Arch Dermatol Res. 2010 Sep;302(7):499-505
pubmed: 20148256
Mol Ther. 2007 Dec;15(12):2070-9
pubmed: 17878899
Mult Scler Relat Disord. 2018 Oct;25:219-226
pubmed: 30114626
Antioxid Redox Signal. 2018 Oct 10;29(11):1064-1073
pubmed: 28934861
J Cancer Res Ther. 2019 Jan-Mar;15(1):108-114
pubmed: 30880764
Brain. 2009 Dec;132(Pt 12):3329-41
pubmed: 19933767
Trends Mol Med. 2001 Mar;7(3):115-21
pubmed: 11286782
Nat Immunol. 2005 Nov;6(11):1123-32
pubmed: 16200070