Plexin B2 Is a Regulator of Monocyte Apoptotic Cell Disassembly.
apoptopodia
apoptosis
apoptotic bodies
apoptotic cell clearance
apoptotic cell disassembly
plexin B2
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
12 11 2019
12 11 2019
Historique:
received:
18
07
2019
revised:
10
09
2019
accepted:
03
10
2019
entrez:
14
11
2019
pubmed:
14
11
2019
medline:
17
9
2020
Statut:
ppublish
Résumé
Billions of cells undergo apoptosis daily and often fragment into small, membrane-bound extracellular vesicles termed apoptotic bodies (ApoBDs). We demonstrate that apoptotic monocytes undergo a highly coordinated disassembly process and form long, beaded protrusions (coined as beaded apoptopodia), which fragment to release ApoBDs. Here, we find that the protein plexin B2 (PlexB2), a transmembrane receptor that regulates axonal guidance in neurons, is enriched in the ApoBDs of THP1 monocytes and is a caspase 3/7 substrate. To determine whether PlexB2 is involved in the disassembly of apoptotic monocytes, we generate PlexB2-deficient THP1 monocytes and demonstrate that lack of PlexB2 impairs the formation of beaded apoptopodia and ApoBDs. Consequently, the loss of PlexB2 in apoptotic THP1 monocytes impairs their uptake by both professional and non-professional phagocytes. Altogether, these data identify PlexB2 as a positive regulator of apoptotic monocyte disassembly and demonstrate the importance of this process in apoptotic cell clearance.
Identifiants
pubmed: 31722200
pii: S2211-1247(19)31314-2
doi: 10.1016/j.celrep.2019.10.014
pii:
doi:
Substances chimiques
Nerve Tissue Proteins
0
PLXNB2 protein, human
0
Plxnb2 protein, mouse
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1821-1831.e3Informations de copyright
Copyright © 2019 The Author(s). Published by Elsevier Inc. All rights reserved.