Micro RNA 181c-5p: A promising target for post-stroke recovery in socially isolated mice.
Inflammation
Post-stroke social isolation
Target validation
miR-181c-5p
miRNA analysis
Journal
Neuroscience letters
ISSN: 1872-7972
Titre abrégé: Neurosci Lett
Pays: Ireland
ID NLM: 7600130
Informations de publication
Date de publication:
10 01 2020
10 01 2020
Historique:
received:
15
07
2019
revised:
28
10
2019
accepted:
02
11
2019
pubmed:
14
11
2019
medline:
15
12
2020
entrez:
14
11
2019
Statut:
ppublish
Résumé
Dysregulation of microRNAs (miRNAs) has been tied to several neurological disorders, including ischemic stroke. It has also been established that social environments can modulate miRNA profiles. We have previously shown that post-stroke social isolation (SI) is linked to poor stroke outcomes and that miR-181c-5p emerged as one of few lead miRNAs that was downregulated in both stroke and SI. Therefore, in this study we examined the potential role of miR-181c-5p mimic in reversing the detrimental effects of post-stroke SI. Two to three-month-old C57BL/6 male mice were pair-housed (PH) for at least two weeks. After two weeks, mice underwent stroke survival surgery using middle cerebral artery occlusion (MCAO) and were randomly assigned to one of two housing conditions: stroke isolation (ST-ISO) or stroke pair-housing with a healthy partner (ST-PH). ST-ISO mice were randomized to receive either miR-181c-5p mimic or a scrambled RNA (7 mg/kg i.v./day×drug) control at 24 h and 48 h after stroke. The effects of miR-181c-5p mimic treatment were evaluated at 1, 3, and 7 days after stroke at histological, behavioral, and biochemical levels. Target genes of miR-181c-5p were then analyzed by qPCR using an RT
Identifiants
pubmed: 31722236
pii: S0304-3940(19)30713-X
doi: 10.1016/j.neulet.2019.134610
pmc: PMC7054848
mid: NIHMS1545349
pii:
doi:
Substances chimiques
MicroRNAs
0
mirn181 microRNA, mouse
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
134610Subventions
Organisme : NINDS NIH HHS
ID : R37 NS096493
Pays : United States
Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.
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